Cat.No. : CSC-SC003242 Host Cell: HEK293 (CHO and other cell types are also available)
| Cat. No. | CSC-SC003242 |
| Description | Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level. |
| Gene | CLDN6 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 (CHO and other cell types are also available) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Size Form | 2 × 10^6 cells / vial |
| Shipping | Dry Ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | CLDN6 claudin 6 [ Homo sapiens ] |
| Gene Symbol | CLDN6 |
| Gene ID | 9074 |
| UniProt ID | P56747 |
| mRNA Refseq | NM_021195.4 |
| Protein Refseq | NP_067018.2 |
| Chromosome Location | 16p13.3 |
| Function | identical protein binding; structural molecule activity; |
| Pathway | Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Cell-cell junction organization, organism-specific biosystem; Hepatitis C, organism-specific biosystem; Hepatitis C, conserved biosystem; |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Recent studies have shown that aberrant expression of tight junction (TJ) proteins is a hallmark of various solid tumors and is considered a promising therapeutic target. Claudin-6 (CLDN6), a member of the TJ transmembrane protein family, is an ideal therapeutic target because it is not expressed in normal adult tissues. Here, researchers found that CLDN6 is highly expressed in uterine cervical adenocarcinoma (ADC), and that elevated CLDN6 expression correlates with lymph node metastasis and lymphovascular invasion, acting as an independent prognostic factor. Shotgun proteomic analysis revealed a significant increase in intercellular adhesion-related proteins and drug metabolism-related proteins (aldo-keto reductase [AKR] family proteins) in CLDN6-overexpressing cells. Furthermore, CLDN6 overexpression enhanced intercellular adhesion and reduced sensitivity to anticancer drugs such as doxorubicin, daunorubicin, and cisplatin. Taken together, these results suggest that aberrant CLDN6 expression enhances the malignant potential and drug resistance of endocervical adenocarcinoma, likely due to enhanced intercellular adhesion and drug metabolism.
Phase contrast microscopy revealed that compared with control cells, CLDN6-overexpressing cells had blurred intercellular boundaries and smoother cell sheet edges (Figure 1A). Scanning electron microscopy revealed an increase in the number of microvilli due to high CLDN6 expression (Figure 1A). In addition to morphological changes, Western blot analysis confirmed increased expression of cell adhesion-related proteins, including CLDN-1 (a TJ-associated protein), E-cadherin (a major adherens junction resident protein), integrin β4 (a focal adhesion-associated protein), and CD44, in CLDN6-overexpressing cells (Figures 1B-D). Next, the researchers investigated the effects of CLDN6 expression on TJ barrier function and cell-matrix adhesion. As expected, compared with control cells, CLDN6-overexpressing cells showed increased transepithelial electrical resistance (an indicator of paracellular ion movement), while CLDN6-overexpressing cells showed decreased paracellular permeability to fluorescein-labeled dextran (Figures 1E, F), indicating that CLDN6 overexpression enhances TJ barrier function. Furthermore, in an adhesion assay that assesses the adhesion between cells and substrates, overexpression of CLDN6 significantly increased the number of adherent cells (Figure 1G). Taken together, these results suggest that CLDN6 overexpression may promote cell adhesion by inducing cell adhesion-related proteins.
Figure 1. Overexpression of claudin-6 (CLDN6) enhances cell adhesion-associated properties of cervical adenocarcinoma cells. (Ito Y, et al., 2022)
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