Human CLDN6 Stable Cell Line - HEK293T

The CLDN6 gene, also known as claudin 6, is located on chromosome 16p13.3 and encodes a member of the claudin family of tight junction proteins. These proteins play a critical role in forming continuous seals around epithelial and endothelial cells, acting as a physical barrier that controls the movement of solutes and water through the paracellular space. CLDN6 is an integral membrane protein with four transmembrane domains and has been identified as an entry cofactor for hepatitis C virus. Its expression is tightly regulated during development and is often aberrantly expressed in various cancers, making it a target of interest for therapeutic development. The Human CLDN6 Stable Cell Line - HEK293T is generated by stably expressing the human CLDN6 gene in the HEK293T background. HEK293T cells are a highly popular derivative of the original HEK293 line, engineered to express the SV40 large T antigen, which allows for high-level protein expression and episomal replication of transfected plasmids carrying the SV40 origin of replication. This combination makes the cell line a robust and reproducible tool for studying CLDN6 biology.

This stable cell line has broad utility across multiple research applications. It serves as a valuable tool for antibody development and screening, particularly for therapeutic antibodies targeting CLDN6-positive tumors such as ovarian, gastric, and lung cancers. Researchers can use the line to characterize antibody binding specificity, affinity, and internalization properties. The cell line is also well suited for functional studies examining the role of CLDN6 in cell adhesion, cell migration, and tumor biology. In drug discovery, it can be employed for high-throughput screening of small molecule inhibitors or biologics that modulate CLDN6 activity. Additionally, the line supports the development and validation of CAR-T cell therapies and bispecific T cell engagers targeting CLDN6. In virology research, given CLDN6’s role as a hepatitis C virus entry factor, the cell line can be used to study viral entry mechanisms and screen for antiviral compounds.