Cat.No. : CSC-SC000319 Host Cell: HEK293 (CHO and other cell types are also available)
| Cat. No. | CSC-SC000319 |
| Description | Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level. |
| Gene | ADORA2A |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 (CHO and other cell types are also available) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Size Form | 2 × 10^6 cells / vial |
| Shipping | Dry Ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | ADORA2A adenosine A2a receptor [ Homo sapiens ] |
| Gene Symbol | ADORA2A |
| Synonyms | RDC8; hA2aR; ADORA2 |
| Gene Description | adenosine A2a receptor |
| Gene ID | 135 |
| UniProt ID | B3KVQ4 |
| mRNA Refseq | NM_000675.4 |
| Protein Refseq | NP_000666.2 |
| Chromosome Location | 22q11.23 |
| Function | G-protein coupled adenosine receptor activity; enzyme binding; protein binding; protein heterodimerization activity; type 5 metabotropic glutamate receptor binding; |
| Pathway | Activation of TRKA receptors, organism-specific biosystem; Adenosine P1 receptors, organism-specific biosystem; Alcoholism, organism-specific biosystem; Alcoholism, conserved biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; |
| MIM | 102776 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Colorectal cancer (CRC) is the third most common disease worldwide and the second most common cause of cancer-related death. ADORA2A has been shown to be highly expressed in many human malignancies. Here, researchers identified ADORA2A through RNA sequencing analysis as playing a significant role in CRC prognosis. Compared to the SW480 cell line, ADORA2A expression is relatively high in the SW620 and HCT116 cell lines. In both SW620 and HCT116 cell lines, ADORA2A knockdown inhibited cell proliferation, migration, and invasion, while ADORA2A overexpression had the opposite effect. Furthermore, ADORA2A expression affected the expression of apoptosis-related proteins (including Bcl-2, Bax, cleaved caspase-3, and cleaved caspase-9) and reduced apoptosis. This process may involve the PI3K/AKT signaling pathway. ADORA2A promotes colorectal cancer progression and inhibits apoptosis through the PI3K/AKT signaling pathway. It may be helpful for the management and treatment of colorectal cancer.
The researchers analyzed colorectal cancer cell lines (CRC) by flow cytometry to investigate whether ADORA2A is involved in apoptosis. Compared with NC cells, the apoptosis rate of ADORA2A-knockdown SW620 and HCT116 cells was significantly increased (Figures 1A-B). In contrast, the apoptosis rate of ADORA2A-overexpressing SW480 cells was reduced (Figure 1C). Furthermore, the expression of apoptosis-related proteins was detected. Transfection with siRNA ADORA2A upregulated the expression of pro-apoptotic proteins (primarily Bax, cleaved caspase3, and cleaved caspase9), while the expression of the anti-apoptotic protein Bcl-2 was downregulated. However, the opposite pattern was observed in ADORA2A-overexpressing SW480 cells. These results suggest that ADORA2A can inhibit apoptosis in colorectal cancer cells.
Figure 1. ADORA2A inhibits apoptosis of CRC cells. (Ran L, et al., 2023)
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