Human ADORA2A Stable Cell Line-HEK293

Name: Human ADORA2A Stable Cell Line-HEK293
SKU: CSC-RG1114
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG1114

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG1114
Description	This cell line is engineered to stably overexpress Human ADORA2A in HEK293 cells.
Target Gene	ADORA2A
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ADORA2A adenosine A2a receptor [ Homo sapiens ]
Gene Symbol	ADORA2A
Synonyms	RDC8; hA2aR; ADORA2
Gene Description	adenosine A2a receptor
Gene ID	135
Uni Prot ID	B3KVQ4
m RNA Refseq	NM_000675.4
Protein Refseq	NP_000666.2
Chromosome Location	22q11.23
Function	G-protein coupled adenosine receptor activity; enzyme binding; protein binding; protein heterodimerization activity; type 5 metabotropic glutamate receptor binding;
Pathway	Activation of TRKA receptors, organism-specific biosystem; Adenosine P1 receptors, organism-specific biosystem; Alcoholism, organism-specific biosystem; Alcoholism, conserved biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem;
MIM	102776

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