Mouse Adora2a Stable Cell Line - CHO-K1

Name: Mouse Adora2a Stable Cell Line - CHO-K1
SKU: CSC-RG1754
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG1754

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG1754
Introduction	This cell line is constructed by transfection of mouse adenosine A2a receptor (Adora2a) into CHO-K1, followed by stable cell selection. The functional activity of Adora2a receptor in this cell llne has been validated through cAMP assay.
Target Gene	Adora2a
Gene Species	Mus musculus (Mouse)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Adora2a adenosine A2a receptor [ Mus musculus ]
Gene Symbol	Adora2a
Synonyms	A2aR; A2AAR; AA2AR
Gene Description	adenosine A2a receptor
Gene ID	11540
Uni Prot ID	Q60613
m RNA Refseq	NM_009630.2
Protein Refseq	NP_033760.2
Chromosome Location	10 B5.3; 10
Function	G-protein coupled adenosine receptor activity; G-protein coupled adenosine receptor activity; G-protein coupled adenosine receptor activity; G-protein coupled receptor activity; alpha-actinin binding; enzyme binding; protein heterodimerization activity; signal transducer activity; type 5 metabotropic glutamate receptor binding;
Pathway	Activation of TRKA receptors, organism-specific biosystem; Adenosine P1 receptors, organism-specific biosystem; Alcoholism, organism-specific biosystem; Alcoholism, conserved biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem;

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