Cat.No. : CSC-RG0005 Host Cell: U2OS
| Cat. No. | CSC-RG0005 |
| Background | Muscarinic acetylcholine receptors are G protein-coupled receptors. M1, M3, M5 receptors couple to G proteins of the Gq/11 family, which activate phospholipase C. M2 and M4 receptors couple to Gi/o-type G proteins that inhibit adenylyl cyclase activity. Muscarinic receptors control many effects of acetylcholine in the central and peripheral nervous system. M1 receptor is known to mediate slow EPSP at the ganglion in the postganglionic nerve, and it is also found in exocrine glands and in the CNS. M1 receptor is thought to be implicated in Alzheimer′s disease. |
| Gene | CHRM1 |
| Gene Species | Homo sapiens (Human) |
| Alias | CHRM1, HM1, M1, M1R |
| Host Cell | U2OS |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: In creating the Human CHRM1 Stable Cell Line-U2OS, the CRISPR-Cas9 system is used to precisely insert or edit the CHRM1 gene. This typically involves designing specific guide RNAs (gRNAs) that are complementary to the CHRM1 gene sequence, guiding the Cas9 enzyme to cut at the target site. Insertion or editing of the CHRM1 gene can be achieved through homology-directed repair (HDR) or non-homologous end joining (NHEJ), resulting in stable expression in U2OS cells.
A: The Human CHRM1 Stable Cell Line-U2OS can be used to study the role of the muscarinic acetylcholine receptor (CHRM1) in neural transmission and neurological diseases. By performing drug screening and functional analysis on these cells, scientists can better understand the role of CHRM1 in cognitive function, memory, and neurological disorders such as Alzheimer's disease.
A: In drug development, the Human CHRM1 Stable Cell Line-U2OS can be used to screen and evaluate potential CHRM1 agonists or antagonists. By monitoring the effects of drugs on the signaling pathways mediated by CHRM1, such as changes in calcium ion levels, the potency and selectivity of drugs can be assessed.
A: The cell line can be used to assess the potential toxicity of drugs on CHRM1-expressing cells. By monitoring cell viability, apoptosis, and cell cycle changes after drug treatment, the safety and side effects of drugs can be evaluated.
A: The cell line can be used to study the combined effects of different drugs on CHRM1 and how they influence the signaling pathways mediated by CHRM1. This is crucial for understanding the efficacy and potential risks of drug combinations.
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