Human CHRM1 Stable Cell Line-U2OS

Name: Human CHRM1 Stable Cell Line-U2OS
SKU: CSC-RG0005
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0005

Host Cell : U2OS Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0005
Background	Muscarinic acetylcholine receptors are G protein-coupled receptors. M1, M3, M5 receptors couple to G proteins of the Gq/11 family, which activate phospholipase C. M2 and M4 receptors couple to Gi/o-type G proteins that inhibit adenylyl cyclase activity. Muscarinic receptors control many effects of acetylcholine in the central and peripheral nervous system. M1 receptor is known to mediate slow EPSP at the ganglion in the postganglionic nerve, and it is also found in exocrine glands and in the CNS. M1 receptor is thought to be implicated in Alzheimer′s disease.
Target Gene	CHRM1
Gene Species	Homo sapiens (Human)
Abbr	U2OS-HuCHRM1
Alias	CHRM1, HM1, M1, M1R
Host Cell	U2OS
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CHRM1 cholinergic receptor, muscarinic 1 [ Homo sapiens ]
Gene Symbol	CHRM1
Synonyms	M1; HM1; M1R
Gene Description	cholinergic receptor, muscarinic 1
GeneID	1128
Uni ProtID	P11229
mRNA Refseq	NM_000738.2
Protein Refseq	NP_000729.2
Chromosome Location	11q13
Function	G-protein coupled acetylcholine receptor activity; drug binding; phosphatidylinositol phospholipase C activity;
Pathway	Amine ligand-binding receptors, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Cholinergic synapse, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem;
MIM	118510

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Customer Q&As

How is the Human CHRM1 Stable Cell Line-U2OS achieved through CRISPR-Cas9 technology?

A: In creating the Human CHRM1 Stable Cell Line-U2OS, the CRISPR-Cas9 system is used to precisely insert or edit the CHRM1 gene. This typically involves designing specific guide RNAs (gRNAs) that are complementary to the CHRM1 gene sequence, guiding the Cas9 enzyme to cut at the target site. Insertion or editing of the CHRM1 gene can be achieved through homology-directed repair (HDR) or non-homologous end joining (NHEJ), resulting in stable expression in U2OS cells.

What are the potential applications of the Human CHRM1 Stable Cell Line-U2OS in neuroscience research?

A: The Human CHRM1 Stable Cell Line-U2OS can be used to study the role of the muscarinic acetylcholine receptor (CHRM1) in neural transmission and neurological diseases. By performing drug screening and functional analysis on these cells, scientists can better understand the role of CHRM1 in cognitive function, memory, and neurological disorders such as Alzheimer's disease.

What is the screening strategy for the Human CHRM1 Stable Cell Line-U2OS in drug development?

A: In drug development, the Human CHRM1 Stable Cell Line-U2OS can be used to screen and evaluate potential CHRM1 agonists or antagonists. By monitoring the effects of drugs on the signaling pathways mediated by CHRM1, such as changes in calcium ion levels, the potency and selectivity of drugs can be assessed.

What is the application of the Human CHRM1 Stable Cell Line-U2OS in drug toxicity studies?

A: The cell line can be used to assess the potential toxicity of drugs on CHRM1-expressing cells. By monitoring cell viability, apoptosis, and cell cycle changes after drug treatment, the safety and side effects of drugs can be evaluated.

What is the value of the Human CHRM1 Stable Cell Line-U2OS in drug-drug interaction studies?

A: The cell line can be used to study the combined effects of different drugs on CHRM1 and how they influence the signaling pathways mediated by CHRM1. This is crucial for understanding the efficacy and potential risks of drug combinations.

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