Human CHRM1-tGFP Stable Cell Line-CHO-K1

Name: Human CHRM1-tGFP Stable Cell Line-CHO-K1
SKU: CSC-RG0024
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0024

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0024
Target Gene	CHRM1
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuCHRM1-tGFP
Alias	CHRM1, M1, HM1, M1R
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CHRM1 cholinergic receptor, muscarinic 1 [ Homo sapiens ]
Gene Symbol	CHRM1
Synonyms	M1; HM1; M1R
Gene Description	cholinergic receptor, muscarinic 1
GeneID	1128
Uni ProtID	P11229
mRNA Refseq	NM_000738.2
Protein Refseq	NP_000729.2
Chromosome Location	11q13
Function	G-protein coupled acetylcholine receptor activity; drug binding; phosphatidylinositol phospholipase C activity;
Pathway	Amine ligand-binding receptors, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Cholinergic synapse, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem;
MIM	118510

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Background

Q & A

Customer Reviews

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13. The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins.

Customer Q&As

What is the specific utility of the Human CHRM1-tGFP Stable Cell Line-CHO in studying muscarinic receptor function?

A: The Human CHRM1-tGFP Stable Cell Line-CHO (Chinese Hamster Ovary) is a valuable tool for investigating the function of the muscarinic acetylcholine receptor subtype 1 (CHRM1). This cell line expresses the CHRM1 receptor fused with a green fluorescent protein (tGFP), which allows for real-time monitoring of receptor activation and localization within the cell. This facilitates detailed studies on receptor trafficking, ligand binding, and downstream signaling pathways, contributing to the development of drugs targeting this receptor.

How does the Human CHRM1-tGFP Stable Cell Line-CHO contribute to the understanding of muscarinic receptor pharmacology?

A: This cell line enables researchers to perform high-throughput screening for compounds that modulate CHRM1 activity. By monitoring the fluorescent signal, researchers can assess the efficacy and potency of various drugs, providing a quantitative measure of their interaction with the receptor. This information is crucial for the development of therapeutic agents targeting muscarinic receptors, particularly in conditions such as Alzheimer's disease and chronic obstructive pulmonary disease (COPD).

What are the challenges associated with maintaining the stability of the Human CHRM1-tGFP Stable Cell Line-CHO over time?

A: One of the challenges is ensuring that the tGFP-tagged CHRM1 receptor remains stably expressed and functional over multiple passages. Genetic drift, selection pressure, and culture conditions can lead to changes in receptor expression levels or function. Regular monitoring and validation of receptor expression and function are necessary to maintain the integrity of the cell line for research purposes.

How does the Human CHRM1-tGFP Stable Cell Line-CHO aid in the study of receptor desensitization and resensitization?

A: The cell line allows for the investigation of receptor desensitization, where prolonged activation leads to a reduced response to agonists, and resensitization, where the receptor regains its responsiveness. By tracking the tGFP fluorescence, researchers can monitor receptor internalization and recycling, providing insights into the dynamic processes that govern receptor function.

In what ways does the Human CHRM1-tGFP Stable Cell Line-CHO facilitate the study of GPCR (G-protein coupled receptor) trafficking?

A: This cell line is instrumental in studying the trafficking of GPCRs, such as CHRM1, from the endoplasmic reticulum to the cell surface, as well as their recycling and degradation pathways. The tGFP tag enables the visualization of receptor movement within the cell, which can be correlated with functional assays to understand the role of trafficking in receptor function and regulation.

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