Cat.No. : CSC-RG0024 Host Cell: CHO-K1
| Cat. No. | CSC-RG0024 |
| Gene | CHRM1 |
| Gene Species | Homo sapiens (Human) |
| Alias | CHRM1, M1, HM1, M1R |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human CHRM1-tGFP Stable Cell Line-CHO (Chinese Hamster Ovary) is a valuable tool for investigating the function of the muscarinic acetylcholine receptor subtype 1 (CHRM1). This cell line expresses the CHRM1 receptor fused with a green fluorescent protein (tGFP), which allows for real-time monitoring of receptor activation and localization within the cell. This facilitates detailed studies on receptor trafficking, ligand binding, and downstream signaling pathways, contributing to the development of drugs targeting this receptor.
A: This cell line enables researchers to perform high-throughput screening for compounds that modulate CHRM1 activity. By monitoring the fluorescent signal, researchers can assess the efficacy and potency of various drugs, providing a quantitative measure of their interaction with the receptor. This information is crucial for the development of therapeutic agents targeting muscarinic receptors, particularly in conditions such as Alzheimer's disease and chronic obstructive pulmonary disease (COPD).
A: One of the challenges is ensuring that the tGFP-tagged CHRM1 receptor remains stably expressed and functional over multiple passages. Genetic drift, selection pressure, and culture conditions can lead to changes in receptor expression levels or function. Regular monitoring and validation of receptor expression and function are necessary to maintain the integrity of the cell line for research purposes.
A: The cell line allows for the investigation of receptor desensitization, where prolonged activation leads to a reduced response to agonists, and resensitization, where the receptor regains its responsiveness. By tracking the tGFP fluorescence, researchers can monitor receptor internalization and recycling, providing insights into the dynamic processes that govern receptor function.
A: This cell line is instrumental in studying the trafficking of GPCRs, such as CHRM1, from the endoplasmic reticulum to the cell surface, as well as their recycling and degradation pathways. The tGFP tag enables the visualization of receptor movement within the cell, which can be correlated with functional assays to understand the role of trafficking in receptor function and regulation.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
