Cat.No. : CSC-RG0065 Host Cell: CHO-K1
| Cat. No. | CSC-RG0065 |
| Background | The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13. |
| Gene | CHRM1 |
| Gene Species | Homo sapiens (Human) |
| Alias | CHRM1, M1, HM1, M1R, MGC30125 |
| Host Cell | CHO-K1 |
| Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: When establishing a Human CHRM1 Stable Cell Line-CHO, gene editing technologies such as CRISPR-Cas9, TALENs (Transcription Activator-Like Effector Nucleases), and ZFNs (Zinc Finger Nucleases) are commonly employed. CRISPR-Cas9 is favored for its high efficiency and flexibility, allowing researchers to precisely insert, delete, or replace specific DNA sequences in the genome to achieve stable integration and expression of the CHRM1 gene.
A: The advantages of using the Human CHRM1 Stable Cell Line-CHO in drug screening and toxicity testing include its ability to mimic human physiological conditions, which makes the research results more clinically relevant. Additionally, the simple genetic background of CHO cell lines, ease of genetic manipulation, and rapid growth rate facilitate quick screening and evaluation of the activity and safety of potential drugs.
A: The Human CHRM1 Stable Cell Line-CHO plays a significant role in neuroscience research, particularly in studying the function and drug action mechanisms of acetylcholine receptors (CHRM1 being one of its subtypes). By expressing CHRM1 in these cells, scientists can model the physiological actions of neurotransmitters, investigate how drugs affect neural signaling, and develop new therapies for neurodegenerative diseases.
A: Assessing the selectivity of drugs for the CHRM1 receptor during drug development typically involves a variety of biochemical and molecular biology techniques. This includes radioligand binding assays, functional assays (such as calcium imaging or patch-clamp techniques), and structure-activity relationship (SAR) analysis. These methods help researchers determine the affinity and mode of action of drugs with the CHRM1 receptor, thereby evaluating their selectivity.
A: Precise regulation of the CHRM1 gene in the Human CHRM1 Stable Cell Line-CHO can be achieved using inducible expression systems, such as the tetracycline-inducible system or the Cre-LoxP system. These systems allow for the activation or repression of CHRM1 gene expression under specific conditions, enabling dynamic studies and drug response analyses.
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