Human CHRM1 Stable Cell Line-CHO-K1

Name: Human CHRM1 Stable Cell Line-CHO-K1
SKU: CSC-RG0065
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0065

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0065
Background	The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13.
Target Gene	CHRM1
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuCHRM1
Alias	CHRM1, M1, HM1, M1R, MGC30125
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CHRM1 cholinergic receptor, muscarinic 1 [ Homo sapiens ]
Gene Symbol	CHRM1
Synonyms	M1; HM1; M1R
Gene Description	cholinergic receptor, muscarinic 1
GeneID	1128
Uni ProtID	P11229
mRNA Refseq	NM_000738.2
Protein Refseq	NP_000729.2
Chromosome Location	11q13
Function	G-protein coupled acetylcholine receptor activity; drug binding; phosphatidylinositol phospholipase C activity;
Pathway	Amine ligand-binding receptors, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Cholinergic synapse, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem;
MIM	118510

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Q & A

Customer Reviews

Customer Q&As

What gene editing technologies are typically used to ensure stable expression of the CHRM1 gene when establishing a Human CHRM1 Stable Cell Line-CHO?

A: When establishing a Human CHRM1 Stable Cell Line-CHO, gene editing technologies such as CRISPR-Cas9, TALENs (Transcription Activator-Like Effector Nucleases), and ZFNs (Zinc Finger Nucleases) are commonly employed. CRISPR-Cas9 is favored for its high efficiency and flexibility, allowing researchers to precisely insert, delete, or replace specific DNA sequences in the genome to achieve stable integration and expression of the CHRM1 gene.

What are the advantages of using the Human CHRM1 Stable Cell Line-CHO in drug screening and toxicity testing?

A: The advantages of using the Human CHRM1 Stable Cell Line-CHO in drug screening and toxicity testing include its ability to mimic human physiological conditions, which makes the research results more clinically relevant. Additionally, the simple genetic background of CHO cell lines, ease of genetic manipulation, and rapid growth rate facilitate quick screening and evaluation of the activity and safety of potential drugs.

What is the role of the Human CHRM1 Stable Cell Line-CHO in neuroscience research?

A: The Human CHRM1 Stable Cell Line-CHO plays a significant role in neuroscience research, particularly in studying the function and drug action mechanisms of acetylcholine receptors (CHRM1 being one of its subtypes). By expressing CHRM1 in these cells, scientists can model the physiological actions of neurotransmitters, investigate how drugs affect neural signaling, and develop new therapies for neurodegenerative diseases.

How is the selectivity of drugs for the CHRM1 receptor assessed when using the Human CHRM1 Stable Cell Line-CHO for drug development?

A: Assessing the selectivity of drugs for the CHRM1 receptor during drug development typically involves a variety of biochemical and molecular biology techniques. This includes radioligand binding assays, functional assays (such as calcium imaging or patch-clamp techniques), and structure-activity relationship (SAR) analysis. These methods help researchers determine the affinity and mode of action of drugs with the CHRM1 receptor, thereby evaluating their selectivity.

How can precise regulation of the CHRM1 gene be achieved in the Human CHRM1 Stable Cell Line-CHO?

A: Precise regulation of the CHRM1 gene in the Human CHRM1 Stable Cell Line-CHO can be achieved using inducible expression systems, such as the tetracycline-inducible system or the Cre-LoxP system. These systems allow for the activation or repression of CHRM1 gene expression under specific conditions, enabling dynamic studies and drug response analyses.

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