Human ADRB2-tGFP Stable Cell Line-CHO-K1

Name: Human ADRB2-tGFP Stable Cell Line-CHO-K1
SKU: CSC-RG0025

Cat.No. : CSC-RG0025 Host Cell: CHO-K1

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RG0025
Gene	ADRB2
Gene Species	Homo sapiens (Human)
Alias	ADRB2, ADRB2R, ADRBR, B2AR, BAR, BETA2AR
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Morphology	Epithelial-like
Stability	Validated for at least 10 passages
Application	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Customer Reviews

This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. The adrenergic receptors are a class of G protein-coupled receptors that are targets of the catecholamines, especially noradrenaline (norepinephrine) andadrenaline (epinephrine). Although dopamine is a catecholamine, its receptors are in a different category. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. Many cells possess these receptors, and the binding of an agonist will generally cause a sympathetic response (i.e. the fight-or-flight response). Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes.

Customer Q&As

How was the Human ADRB2-tGFP Stable Cell Line-CHO created, and what is its unique utility in studying the function of β-adrenergic receptors?

A: The Human ADRB2-tGFP Stable Cell Line-CHO was created by stably transfecting the human β2-adrenergic receptor (ADRB2) gene fused with the green fluorescent protein (tGFP) gene into CHO (Chinese hamster ovary) cells. This cell line allows researchers to track and quantify the expression and function of ADRB2 receptors by observing the fluorescence of GFP, which is crucial for studying how drugs interact with ADRB2 and its role in cellular signaling pathways.

What is the application of the Human ADRB2-tGFP Stable Cell Line-CHO in drug screening and toxicity testing?

A: This cell line is commonly used in high-throughput screening (HTS) and drug discovery processes to assess the affinity and selectivity of potential drugs for the ADRB2 receptor. Additionally, by monitoring the expression of GFP and cell viability, the potential toxic effects of drugs can be evaluated, which is essential for early drug safety assessment.

How does the Human ADRB2-tGFP Stable Cell Line-CHO assist researchers in understanding signal transduction pathways in cellular signaling research?

A: By observing the dynamic changes of GFP following the activation of ADRB2 receptors, researchers can track and quantify signal transduction events, such as receptor phosphorylation, internalization, and interactions with downstream effector molecules. This helps reveal the mechanisms of action of ADRB2 receptors within cellular signaling networks.

What is the role of the Human ADRB2-tGFP Stable Cell Line-CHO in studying the functional differences between receptor isoforms?

A: By comparing the expression and function of different ADRB2 receptor isoforms (such as ADRB1 and ADRB2) in CHO cells, researchers can uncover differences in structure and function between these receptors, which is essential for understanding the significance of receptor diversity in physiological and pathological conditions.

How is the Human ADRB2-tGFP Stable Cell Line-CHO used to study conformational changes in G protein-coupled receptors (GPCRs)?

A: By combining fluorescence resonance energy transfer (FRET) technology, researchers can monitor the conformational changes of ADRB2 receptors during activation, providing in-depth insights into the mechanisms of GPCR activation.

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