Cat.No. : CSC-RG0039 Host Cell: HEK293
| Cat. No. | CSC-RG0039 |
| Background | This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. |
| Gene | ADRB2 |
| Gene Species | Homo sapiens (Human) |
| Alias | ADRB2, ADRB2R, ADRBR, B2AR, BAR, BETA2AR |
| Host Cell | HEK293 |
| Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human ADRB2-SNAP Stable Cell Line-HEK293 is crucial for GPCR research as it allows for the study of the beta-2 adrenergic receptor (ADRB2), a well-characterized GPCR. This cell line enables researchers to investigate the structure-function relationship, ligand binding, and signaling pathways of ADRB2, which are essential for understanding diseases related to GPCR dysfunction.
A: The SNAP tag is a small protein tag that can be fused to the C-terminus of the ADRB2 receptor. It enables the covalent attachment of a wide range of biotin derivatives, fluorescent dyes, or affinity probes through a unique chemistry. This allows for the specific labeling and visualization of the receptor within the cell, which is invaluable for studying its subcellular localization and trafficking.
A: The Human ADRB2-SNAP Stable Cell Line-HEK293 serves as a valuable platform for high-throughput screening of compounds that modulate ADRB2 activity. By assessing the effects of these compounds on receptor function, researchers can identify potential drug candidates that may lead to the development of new treatments for diseases such as asthma, chronic obstructive pulmonary disease (COPD), and heart failure.
A: The Human ADRB2-SNAP Stable Cell Line-HEK293 is distinguished by its stable expression of the ADRB2 receptor with the SNAP tag. This engineered cell line provides a consistent and controlled environment for studying the receptor, which is not typically found in non-engineered HEK293 cells. The SNAP tag also allows for unique labeling and detection methods that are not possible with other cell lines.
A: While the Human ADRB2-SNAP Stable Cell Line-HEK293 is specifically engineered for the study of ADRB2, it can serve as a model system for studying other GPCRs, particularly when investigating common signaling pathways or mechanisms of action. However, for detailed studies on specific GPCRs, it would be necessary to generate similar stable cell lines expressing those receptors.
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