Cat.No. : CSC-RG0058 Host Cell: CHO-K1
| Cat. No. | CSC-RG0058 |
| Background | This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. |
| Gene | ADRB2 |
| Gene Species | Homo sapiens (Human) |
| Alias | ADRB2, ADRB2R, ADRBR, B2AR, BAR, BETA2AR |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Optimizing growth conditions for Human ADRB2 Stable Cell Line-CHO involves several factors. These include selecting the appropriate culture medium, such as F12K or DMEM, which should be supplemented with FBS or other serum components to provide necessary growth factors. The pH and temperature should be maintained at physiological levels (pH 7.2-7.4 and 37°C). Additionally, the cell density and passage number should be controlled to avoid overgrowth or senescence, which can affect the stability of the ADRB2 receptor expression.
A: The response of Human ADRB2 Stable Cell Line-CHO to agonists and antagonists can be assessed through various assays, such as cAMP accumulation, cell proliferation, or gene expression. The cells will typically show an increase in cAMP levels in response to agonists like isoproterenol, and this response can be inhibited by antagonists such as propranolol. The specific response profile can be used to evaluate the efficacy and selectivity of new drugs targeting the ADRB2 receptor.
A: Stability challenges include genetic drift, which can lead to changes in receptor expression levels or function. Additionally, the cells may undergo clonal selection, where certain subpopulations with altered receptor expression become dominant. Regular monitoring and characterization of the cells, as well as periodic re-establishment from frozen stocks, can help maintain the desired phenotype.
A: The cells can be utilized in high-throughput screening by setting up assays that measure the activity of the ADRB2 receptor in response to potential drug candidates. This could involve measuring changes in cAMP levels, calcium flux, or other downstream signaling events. The cells can be plated in multi-well plates, and automated systems can be used to apply compounds and measure their effects.
A: By incorporating techniques such as liquid chromatography-mass spectrometry (LC-MS) to measure drug concentrations in the cell culture medium, and functional assays to measure the biological response, the pharmacokinetic (PK) and pharmacodynamic (PD) profiles of drugs can be studied. This can provide insights into drug absorption, distribution, metabolism, and excretion (ADME) properties, as well as the relationship between drug concentration and effect.
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