Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EROT0328
| Cat. No. | EROT0328 |
| Source | Deinococcus radiophilus |
| Recognition Site | TTT↓AAA |
| Size | 10000 Units;2000 Units |
| Unit Definition | One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour in a total reaction volume of 50 μl. |
| Reaction Buffer | 20 mM Tris-Acetate (pH 7.5 at 37 °C) 50 mM potassium acetate 10 mM magnesium acetate 1 mM dithiothreitol 100 μg/ml bovine serum albumin |
| Reaction Conditions | Incubation at 37 °C |
| Storage | 50 mM Tris-HCl (pH 7.4 at 4 °C) 50 mM NaCl 0.1 mM EDTA 1 mM dithiothreitol 500 μg/ml bovine serum albumin 0.1 mM phenylmethylsulfonyl fluoride 50% (v/v) glycerol |
A: DraI restriction endonuclease recognizes the TTT^AAA site and exhibits optimal cutting efficiency at 37°C in Tango buffer.
A: DraI is at a concentration of 10U/μl, so a total of 1500U is 150μl.
A: DraI should be stored at -20°C, with a tolerance of ±5°C.
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