Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EROT0335
| Cat. No. | EROT0335 |
| Recognition Site | GGTCTC(1/5)↓ |
| Size | 1000 Units;2000 Units |
| Reaction Buffer | 10mM Tris-HCl (pH 7.5 at 37 °C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSA |
| Reaction Conditions | Incubate at 37 °C |
| Storage | 10mM Tris-HCl (pH 7.4 at 25 °C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol |
A: The buffer solution contains: 10 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 50 mM NaCl, and 0.1 mg/mL BSA.
A: The enzyme is provided at a concentration of 10 U/μL, and the volume of the enzyme varies depending on the purchased unit (U) of the product.
A: The enzyme cleavage site is: GGTCTC(1/5)↓.
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We successfully constructed the PGEX-argB expression vector by using this enzyme for site-specific cleavage.
After adding the system according to the instructions, the plasmid can be completely digested at 37°C for 1-2 hours.
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