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FspBI

For research use only. Not intended for any clinical use.
Cat.No.
EROT0351
Reaction Buffer
33mM Tris-acetate (pH7.9 at 37 °C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
Reaction Conditions
Incubate at 37 °C
Recognition Site
C↓TAG
Size/Form
5000 Units;10000 Units
Storage
10mM Tris-HCl (pH 7.4 at 25 °C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

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Q & A

Customer Reviews

Customer Q&As
What is the volume of 500U packaging?

A: TheFspBI comes in a volume of 500U, which is equivalent to 50μl.

How sensitive is the FspBI to methylation?

A: The FspBI is not sensitive to Dam methylation, Dcm methylation, or CpG methylation.

Are there any precautions for use?

A: FspBI cutting can be impaired due to overlapping Dam methylation. To avoid Dam methylation, please use dam-, dcm- strains, such as GM2163.

What are the optimal conditions for the FspBI reaction?

A: The FspBI recognizes the C^TAG site and shows optimal cleavage efficiency at 37°C in Tango buffer.

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