Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EROT0351
| Cat. No. | EROT0351 |
| Recognition Site | C↓TAG |
| Size | 5000 Units;10000 Units |
| Reaction Buffer | 33mM Tris-acetate (pH7.9 at 37 °C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA |
| Reaction Conditions | Incubate at 37 °C |
| Storage | 10mM Tris-HCl (pH 7.4 at 25 °C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol |
A: TheFspBI comes in a volume of 500U, which is equivalent to 50μl.
A: The FspBI is not sensitive to Dam methylation, Dcm methylation, or CpG methylation.
A: FspBI cutting can be impaired due to overlapping Dam methylation. To avoid Dam methylation, please use dam-, dcm- strains, such as GM2163.
A: The FspBI recognizes the C^TAG site and shows optimal cleavage efficiency at 37°C in Tango buffer.
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