Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EROT0385
| Cat. No. | EROT0385 |
| Recognition Site | TGG↓CCA |
| Size | 200 Units;400 Units |
| Reaction Buffer | 10mM Tris-HCl (pH 8.5 at 37 °C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA |
| Reaction Conditions | Incubate at 37 °C |
| Storage | 10mM Tris-HCl (pH7.4 at 25 °C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol |
A: MlsI can be used for molecular cloning, gene typing, Southern blotting, and more.
A: To avoid Dcm methylation, use dam- or dcm- strains, such as GM2163.
A: MlsI recognizes the TGG^CCA site, and its isoschizomers include BalI, MluNI, MscI, Msp20I, among others.
A: MlsI is not sensitive to CpG methylation and will not be methylated.
A: It is sensitive to Dcm methylation, insensitive to Dam methylation, and insensitive to CpG methylation.
A: Our enzyme activity assays use λ phage DNA (dcm-) (#SD0021).
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