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MlsI

For research use only. Not intended for any clinical use.
Cat.No.
EROT0385
Reaction Buffer
10mM Tris-HCl (pH 8.5 at 37 °C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
Reaction Conditions
Incubate at 37 °C
Recognition Site
TGG↓CCA
Size/Form
200 Units;400 Units
Storage
10mM Tris-HCl (pH7.4 at 25 °C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

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Q & A

Customer Reviews

Customer Q&As
What experiments can MlsI be used for?

A: MlsI can be used for molecular cloning, gene typing, Southern blotting, and more.

How can methylation with MlsI be avoided?

A: To avoid Dcm methylation, use dam- or dcm- strains, such as GM2163.

Does MlsI have other isoschizomers or heterospecific enzymes?

A: MlsI recognizes the TGG^CCA site, and its isoschizomers include BalI, MluNI, MscI, Msp20I, among others.

Is MlsI affected by CpG methylation?

A: MlsI is not sensitive to CpG methylation and will not be methylated.

What methylations is MlsI sensitive or insensitive to?

A: It is sensitive to Dcm methylation, insensitive to Dam methylation, and insensitive to CpG methylation.

Which bacterial strain is used for enzyme activity assays?

A: Our enzyme activity assays use λ phage DNA (dcm-) (#SD0021).

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