SatI

Name: SatI
SKU: EROT0458

For research use only. Not intended for any clinical use.

Cat.No.

EROT0458

Reaction Buffer

10mM Tris-HCl (pH7.5 at 37 °C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSA

Reaction Conditions

Incubate at 37 °C

Recognition Site

GC↓NGC

Size/Form

200 Units

Storage

10mM Tris-HCl (pH7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Publications

Q & A

Customer Reviews

Customer Q&As

How sensitive is SatI to methylation?

A: SatI is sensitive to CpG methylation, insensitive to Dam methylation, and insensitive to Dcm methylation.

How many recognition sites does SatI have?

A: When using SatI for restriction digestion, there are 380 cutting sites.

What is the compatible buffer for SatI?

A: SatI enzyme belongs to the high-end enzymes of fast restriction endonucleases and exhibits 100% activity in both universal FastDigest buffer and FastDigest Green reaction buffer.

Is SatI sensitive to heat inactivation?

A: SatI enzyme is sensitive to thermal inactivation.

What is the optimal reaction temperature for SatI?

A: The optimal reaction temperature for SatI enzyme is 37°C.

How should SatI be stored?

A: SatI enzyme should be stored at -20°C.

What are the possible reasons for incomplete or failed digestion when using SatI?

A: The main reasons for the failure of DNA digestion reactions include the presence of inhibitory agents in the template DNA, such as phenol, chloroform, detergents, ethanol, excessive salt, EDTA, etc.

Is there an alternative method to inactivate Thermo Scientific restriction enzymes without using phenol/chloroform when heat inactivation is not suitable?

A: To remove enzymes without using phenol/chloroform, we recommend using silica column purification.

Does the buffer for SatI contain BSA?

A: For convenience, both traditional restriction enzyme buffers and FastDigest universal buffers contain BSA. This eliminates the need to add BSA in a separate step.

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