Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EROT0437
| Cat. No. | EROT0437 |
| Source | Pseudomonas inaequalis |
| Recognition Site | A↓CCGGT |
| Size | 2500 Units;500 Units |
| Unit Definition | One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour in total reaction volume of 50 μl. |
| Reaction Buffer | 20mM Tris-HCl (pH 7.0 at 37 °C) 5 mM MgCl 250 mM KCl 1 mM dithiothreitol 0.1 mg/ml bovine serum albumin |
| Reaction Conditions | Incubation at 37 °C |
| Storage | 20 mM Tris-HCl (pH 7.5 at 4 °C) 0.1 mM EDTA 100 mM NaCl 10 mM β-mercaptoethanol 0.05% (v/v) Triton X-100 50% (v/v) glycerol |
A: Yes, the typical digestion time is 1-16 hours at 37°C, and with well-calculated timing, overnight digestion is possible.
A: It depends on individual demand, generally, larger packaging is more cost-effective.
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