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Cat.No.
EROT0437
Recognition Site
A↓CCGGT
Source Description
Pseudomonas inaequalis
Unit Definition
One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour in total reaction volume of 50 μl.
Reaction Conditions
Incubation at 37 °C
Reaction Buffer
20mM Tris-HCl (pH 7.0 at 37 °C) 5 mM MgCl 250 mM KCl 1 mM dithiothreitol 0.1 mg/ml bovine serum albumin
Size
2500 Units;500 Units
Storage Buffer
20 mM Tris-HCl (pH 7.5 at 4 °C) 0.1 mM EDTA 100 mM NaCl 10 mM β-mercaptoethanol 0.05% (v/v) Triton X-100 50% (v/v) glycerol
Storage Conditions
Store at -20 °C.

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