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PinAI

For research use only. Not intended for any clinical use.

Cat. No. :   EROT0437

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Product Information

Cat. No. EROT0437
Source Pseudomonas inaequalis
Recognition Site A↓CCGGT
Size 2500 Units;500 Units
Unit Definition One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour in total reaction volume of 50 μl.
Reaction Buffer 20mM Tris-HCl (pH 7.0 at 37 °C) 5 mM MgCl 250 mM KCl 1 mM dithiothreitol 0.1 mg/ml bovine serum albumin
Reaction Conditions Incubation at 37 °C
Storage 20 mM Tris-HCl (pH 7.5 at 4 °C) 0.1 mM EDTA 100 mM NaCl 10 mM β-mercaptoethanol 0.05% (v/v) Triton X-100 50% (v/v) glycerol
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Can the enzyme PinAI be used for overnight digestion?

A: Yes, the typical digestion time is 1-16 hours at 37°C, and with well-calculated timing, overnight digestion is possible.

How to purchase more cost-effectively?

A: It depends on individual demand, generally, larger packaging is more cost-effective.

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