Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : VET1263
| Cat. No. | VET1263 |
| Host Cell | plant, Escherichia coli |
| Resistance | Kanamycin |
| Vector Type | Expression Vector |
| Vector Size | 10379 bp |
The pEarleyGate 301 is a high load promoterless plant transformation vector integrated with a C-terminal Hemagglutinin tag, also known as HA tag. Its length is of 10,379 base pairs and the 5' primer is the 35S promoter, with the sequence being CTATCCTTCGCAAGACCCTTC. The vector features a high copy number, making it possible for a high quantity of the vector to be present in each cell, thereby enhancing the scope of vector chains.
The pEarleyGate 301 uses the selectable marker of Phosphinothricin and presents constitutive expression. The majority of its applications are done in Agrobacterium and plant cells, giving it relevance in the field of plant biotechnology and genetic engineering. This vector comes with a kanamycin resistance, which helps in the selection and maintenance of the transformed cells. It is a non-viral vector, which makes it safe for in vivo experiments and plant gene transformation. The pEarleyGate 301 is designed with a C-terminal HA tag to encourage understanding about protein function, confirm a protein’s presence or absence and monitor levels of protein expression at various stages.
pEarleyGate 301 vector is a gene delivery tool commonly used in plant biotechnology, genetic engineering and research. Here are some of the potential applications of the pEarleyGate 301 vector:
Gain-of-Function Studies: By introducing a specific plant gene into the pEarleyGate 301 vector, researchers can overexpress the gene to observe the physiological changes in the plant and understand its potential functions.
Protein Localization: The pEarleyGate 301 vector can be used to study the intracellular localization of proteins in plants. By fusing the target protein with a fluorescent reporter gene, the location of the protein can be easily viewed under a fluorescent microscope.
Functional Genomic Studies: This vector is particularly useful for high-throughput functional genomics. By constructing a plant overexpression library using the pEarleyGate 301 vector, researchers can conduct a large-scale screening to identify the roles of numerous genes.
Investigation of Plant Molecular Mechanisms: The vector can help provide insights into plant molecular procedures such as photosynthesis, plant defense mechanisms, and plant-microbe interactions.
A: This vector allows for expression of genes from a promoter of choice rather than from the enhanced 35S promoter.
A: pEarleyGate 301 vector is a promoterless plant transformation vector with a C-terminal HA tag.
A: pEarleyGate 301 vector allows for the expression of genes from a promoter of choice rather than from the enhanced 35S promoter.
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The pEarleyGate 301 vector is an impressive tool for plant biologists. Its innovative design allows for efficient cloning and plant transformation, significantly reducing the amount of time needed to complete these processes.
Having used the pEarleyGate 301 vector in my lab, I can vouch for its high transformation efficiency. It is a powerful resource for the visualization of plant proteins.
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