mRFP AAV (Serotype 9)

Name: mRFP AAV (Serotype 9)
SKU: AAV00107Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AAV00107Z

Serotype : AAV Serotype 9 Storage : -80 ℃

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Cat. No.	AAV00107Z
Description	mRFP AAV (Serotype 9) is the serotype 9 AAV which expresses mRFP under the CMV promoter. mRFP AAV (Serotype 9) express a monomeric red fluorescent protein mutant from Discosoma (DsRed) , overcome the tetramerization which is toxic to cells. mRFP can be used as a reporter in target cells. Used as a control.
Product Type	Adeno-associated virus
Gene	mRFP
Serotype	AAV Serotype 9
Reporter	mRFP
Applications	1. Determination of optimal MOI (multiplicity of infection), administration methods etc. 2. Detection of the infection efficiency of the AAV serotype against a specific cell type or tissue. 3. Using reporter genes to visualize the distribution and expression of AAV vectors in live animals, helping assess the biodistribution and persistence of gene delivery.
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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Adeno-associated virus (AAV) is a small, nonenveloped virus with a single-stranded DNA genome flanked by two inverted terminal repeats (ITRs). It was originally isolated from an adenoviral preparation and is a "defective" virus in that it requires co-infection with a helper virus (such as adenovirus or herpes virus) for efficient replication.

Due in part to its lack of pathogenicity, limited immunogenicity, and ability to trigger long-term gene expression in postmitotic tissues even in the absence of genomic integration, AAV is considered one of the most promising gene delivery vectors. In 2012, clinical treatment for lipoprotein lipase deficiency was approved in Europe, the first gene therapy approved in the Western world. A collection of AAV serotypes and variants exhibit broad but distinct tissue and cell tropisms. For cardiac gene delivery, the AAV serotype 9 (AAV9) has clearly emerged as the most potent serotype, at least in rodents, when delivered systemically and in pigs and possibly in dogs when delivered regionally.

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Beyond the product quality, the customer service team has been incredibly responsive and helpful. They are always ready to assist with any questions or troubleshooting, making the entire purchasing and application process much more pleasant and stress-free.

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2020-01-08

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mRFP AAV (Serotype 9)

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