Cat.No. : CSC-SC006776-MNH Host Cell: HEK293
| Cat. No. | CSC-SC006776-MNH |
| Description | This cell line is engineered to stably overexpress human codon optimized monkey guanylate cyclase 2C (GUCY2C). |
| Gene | GUCY2C |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Mutations in the GUCY2C gene, which codes for a receptor essential for activating the CFTR protein via cGMP production, have been shown to cause meconium ileus, a kind of intestinal blockage in neonates. Using frequently utilized HEK293 cells—often used for protein expression studies—they investigated the functional ramifications of certain GUCY2C mutations by transfecting both wild-type and mutant GUCY2C constructions in their work This method showed that the receptor's existence on the cell surface stays unaltered despite mutations, suggesting a possible target for next therapeutic intervention research.
Figure 1. The researchers employed stable HEK293 cell lines expressing equal levels of wild-type and mutant GUCY2C to analyze the functional effects of the c.1160A>G mutation on receptor activity. (Romi H, et al., 2012)
Creative Biogene's Monkey GUCY2C Stable Cell Line - HEK293 provides a reliable platform for similar research applications. With the capacity to stably express GUCY2C, this cell line allows researchers to study the receptor's functionality, test potential inhibitors, and evaluate the effects of various mutations.
A: The GUCY2C gene encodes for the guanylate cyclase C (GC-C) protein, which is a membrane-bound receptor. GC-C is primarily expressed in intestinal cells and plays a key role in the regulation of intestinal motility and fluid secretion in response to the gut peptide hormone guanylin and heat-stable enterotoxin.
A: The GUCY2C gene product, guanylate cyclase C, is involved in the intestinal response to luminal contents. Upon stimulation by guanylin or heat-stable enterotoxin, GC-C activates the cGMP signaling pathway, leading to enhanced intestinal motility and secretion. This process is important for the regulation of bowel movements and the elimination of pathogens.
A: Yes, mutations in the GUCY2C gene can lead to functional deficiencies in guanylate cyclase C, resulting in intestinal disorders. For example, mutations in this gene have been associated with familial diarrhea and other gastrointestinal conditions, such as chronic idiopathic constipation and functional gastrointestinal disorders.
A: The expression of the GUCY2C gene in intestinal cells is regulated by various factors, including the presence of luminal contents, hormonal signals, and the physiological state of the gut. Transcription factors and epigenetic modifications also play a role in controlling the expression of GUCY2C, ensuring that guanylate cyclase C is produced in the appropriate cells and at the right levels to maintain intestinal homeostasis.
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The Monkey GUCY2C Stable Cell Line - HEK293 exhibits high homogeneity, ensuring consistent genetic and phenotypic characteristics across the cell population. This uniformity is critical for reliable and reproducible experimental results, as it minimizes variations that could potentially confound research findings.
The Monkey GUCY2C Stable Cell Line - HEK293 has strong clinical relevance, serving as an important model for studying the role of GUCY2C in various diseases, particularly those related to the endocrine system. Its relevance to human biology makes it an invaluable asset for advancing medical research and developing novel therapeutic strategies.
The Monkey GUCY2C Stable Cell Line - HEK293 is readily available for distribution and sharing among research institutions, promoting collaboration and knowledge exchange in the scientific community. This accessibility enhances the dissemination of valuable research tools and facilitates the advancement of scientific discoveries.
The production and use of the Monkey GUCY2C Stable Cell Line - HEK293 adhere to strict ethical guidelines and safety standards. This ensures responsible research practices and minimizes potential risks associated with the use of genetically modified cell lines, protecting both researchers and the broader community from unintended consequences.
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