Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RR0567
Host Cell : Daudi Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RR0567 |
| Description | This cell line is engineered to stably overexpress luciferase reporter gene in Daudi cells. |
| Target Gene | Luc |
| Host Cell | Daudi |
| Host Cell Species | Homo sapiens (Human) |
| Applications |
1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The Mouse Fap stable cell line CT26 is a genetically modified cell line designed to stably express Fibroblast Activation Protein (Fap), a cell surface glycoprotein associated with tumor growth and progression. The Fap gene, which codes for Fap, is expressed in stromal cells across a range of organs and is involved in tissue repair and extracellular matrix remodeling.
Derived from the CT26 mouse colon cancer cell line, this stable cell line is an invaluable resource for researching the relationship between tumor cells and the surrounding environment. Under typical culture conditions, CT26 cells can develop in monolayers because they have an epithelial shape and stick to culture surfaces.
The Luc reporter cell line - Daudi is a valuable tool in various fields of biomedical research due to its unique properties and capabilities. Here are some of the key applications:
Tumor growth and metastasis studies: This cell line can be used to monitor tumor growth and metastatic spread in vivo via bioluminescent imaging, providing real-time insights into cancer progression.
Drug efficacy testing: Researchers can use this cell line to assess the effectiveness of anticancer drugs by measuring changes in bioluminescence, which correlates with cell viability and proliferation.
Immune cell-cancer cell interactions: Bioluminescent properties allow tracking of interactions between cancer cells and immune cells, aiding in the development and optimization of immunotherapy strategies.
Cytotoxicity assays: This cell line can be used in cytotoxicity assays to assess the destructive effects of immune cells or therapeutic agents on cancer cells.
Pathway analysis: Researchers can use this cell line to study signal transduction pathways. By linking luciferase expression to specific signaling events, pathway activation and inhibition in response to various stimuli can be studied.
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The Luc Reporter Cell Line - Daudi has greatly improved the consistency of our assays. The reliability of the bioluminescent signal ensures that we obtain reproducible results every time.
I found the Luc Reporter Cell Line very easy to operate, even for less experienced lab members.
The sensitivity and dynamic range of the Luc Reporter Cell Line - Daudi are excellent. It has enabled us to detect subtle changes in gene expression that were previously difficult to observe in other cell lines.
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