Cat.No. : CSC-RO0675 Host Cell: CT26
| Cat. No. | CSC-RO0675 |
| Description | This cell line is engineered to stably overexpress the fusion protein of Firefly luciferase and human CXADR [CXADR Ig-like cell adhesion molecule]. |
| Gene | Luc-CXADR |
| Host Cell | CT26 |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Using a CXADR stable cell line, the researchers sought to create a novel anti-tumor antibody. They identified the coxsackie virus and adenovirus receptor (CXADR) as a target by performing a signal sequence trap by retrovirus-mediated expression. One monoclonal antibody, 6G10A, was found to strongly limit the growth of subcutaneous and orthotopic xenografts of human prostate cancer cells in vivo. These antibodies were produced against human CXADR. Other cancer xenografts that expressed CXADR, such as colorectal and pancreatic cancer cells, were also suppressed by 6G10A. The dependence of 6G10A's anti-tumor efficacy on CXADR expression was verified by tests using both knockdown and overexpression. Mechanistic investigations demonstrated that 6G10A acted via complement- and antibody-dependent cellular cytotoxicity.
Figure 1. The researchers investigated CXADR protein levels in various normal and tumor tissues using a commercial CXADR antibody. (Kawada M, et al., 2017)
If you want to simplify the process of similar experiments, Creative Biogene's Luc-Human-CXADR stable cell line - CT26 provides a valuable shortcut. By providing a pre-established stable cell line expressing CXADR, fully equipped with bioluminescence tracking capabilities, researchers can efficiently evaluate the efficacy and mechanism of potential therapeutic antibodies such as 6G10A. This ready-to-use cell line enables rapid experimentation, from validating the efficacy of antibodies in various cancer types to elucidating their mechanisms of action, ultimately accelerating cancer research and drug development.
A: Luciferase expression in Luc-Human CXADR Stable Cell Line-CT26 has been tested over a long period of time and the expression level remains stable. We conducted multiple passage experiments, and the luciferase activity was quantitatively analyzed using a fluorescence detector, and the results showed no significant decrease.
A: CXADR expression was verified by qPCR and Western Blot. The qPCR results showed that there was no significant difference in the expression levels of CXADR mRNA in different passages. Western Blot results also showed that the expression level of CXADR protein in each passage was consistent. Specific experimental data can be provided according to customer needs.
A: We conducted strict optimization of the dual-gene expression system to ensure independent and stable expression of luciferase and CXADR. Experimental results show that the expressions of the two do not interfere with each other. We verified it through independent luciferase activity measurements and CXADR functional experiments to ensure the reliability of the experimental data.
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This is a very strong cell line. It resembles a secret weapon for studying viral outbreaks. The versatility of the CT26 background is enhanced by the fusion protein, which facilitates infection tracking.
Researchers studying viral pathogenesis and cancer biology can benefit greatly from the use of the Luc-Human-CXADR Stable Cell Line. Its fusion protein makes it possible to track viral infections non-invasively, and the CT26 background provides a well-known model system for research on cancer. This cell line produces consistent results and simplifies experimental workflows thanks to its robust performance and ease of use.
This cell line is revolutionary! It is comparable to knowing a virus study cheat code. It's very cool that you can see what's happening in real time.
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