Cat.No. : CSC-RO0494 Host Cell: CHO-K1
| Cat. No. | CSC-RO0494 |
| Description | This cell line is derived from CHO-K1 and is engineered to stably overexpress Human TPBG. |
| Gene | TPBG |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
TPBG induces metastasis in lung adenocarcinoma cells
Since researchers found that TPBG was highly expressed in lung adenocarcinoma and positively correlated with the expression of LINC00342,they then investigated the function of TPBG in lung adenocarcinoma metastasis.Q-PCR analyses showed that TPBG silencing promoted the expression of CDH1,whereas VIM and SN4I1 were suppressed;in contrast,TPBG overexpression suppressed CDHf,whereas VIM and SNAI1 were elevated.Furthermore,TPBG siRNA inhibited the invasive ability of cells,whereas ecotopic TPBG expression enhanced the invasive ability of cells.
Figure 1.TPBG induced metastasis of lung adenocarcinoma cells.(A)and(B)Western blot analysis of TPBG expression after transfected with TPBG siRNA and overexpression vector.(c)Q-PCR analysis of EMT markers after silencing TPBG in cells.(D)Q-PCR analysis of EMT markers after ectopically expressing TPBG in cell.The invasion ability of A549 cells was evaluated by transwell assay in silencing(E)and overexpressing TPBG cell(F).(Hang Su,et al.,2022)
A: The TPBG gene encodes for the Tissue Polypeptide Aligned with Glycoprotein Hormones, which is a glycoprotein hormone involved in the regulation of metabolism and reproduction. It's also known as thyroid stimulating hormone beta subunit (TSHB).
A: The TPBG gene is responsible for the production of the beta subunit of glycoprotein hormones, which includes thyroid-stimulating hormone (TSH), luteinizing hormone (LH), and follicle-stimulating hormone (FSH). These hormones play critical roles in regulating the thyroid gland, gonads, and the menstrual cycle.
A: Yes, mutations in the TPBG gene can lead to hypothyroidism due to impaired production or function of thyroid-stimulating hormone. This condition may result in developmental issues, intellectual disability, and other metabolic disorders.
A: The expression of the TPBG gene is regulated by a complex interplay of transcription factors and signaling pathways. For instance, the hypothalamic-pituitary-gonadal axis controls the expression of TPBG in the pituitary gland in response to the levels of sex hormones and feedback loops.
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The Human TPBG Stable Cell Line - CHO-K1 exhibits high knockout efficiency, ensuring effective silencing of the TPBG gene. This efficiency is crucial for accurately studying the role of TPBG in various biological processes and its implications in disease development.
This cell line maintains excellent genetic stability, with the knockout phenotype remaining consistent over numerous passages. This stability is essential for reliable experimental results and the longevity of the cell line for long-term studies.
The Human TPBG Stable Cell Line - CHO-K1 displays robust growth kinetics and a morphology similar to wild-type CHO-K1 cells. This similarity is beneficial for comparative studies, as it minimizes the impact of confounding factors and allows for a clear interpretation of the specific effects resulting from the TPBG knockout.
The Human TPBG Stable Cell Line - CHO-K1 serves as a valuable tool for functional studies, enabling researchers to investigate the role of TPBG in gene regulation and cellular processes. Its stable knockout phenotype provides a reliable platform for understanding the functional consequences of TPBG deletion and its potential implications in disease pathogenesis.
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