Human SRD5A1 Stable Cell Line - HEK293

Name: Human SRD5A1 Stable Cell Line - HEK293
SKU: CSC-SC015138-1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC015138-1

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC015138-1
Description	This cell line is engineered to stably express human steroid 5 alpha-reductase 1 (SRD5A1) in HEK293 cells.
Target Gene	SRD5A1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SRD5A1 steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) [ Homo sapiens ]
Gene Symbol	SRD5A1
Synonyms	S5AR 1
Gene Description	steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
GeneID	6715
Uni ProtID	P18405
mRNA Refseq	NM_001047.2
Protein Refseq	NP_001038.1
Chromosome Location	5p15
Function	3-oxo-5-alpha-steroid 4-dehydrogenase activity; NADPH binding; amide binding; electron carrier activity; progesterone 5-alpha-reductase activity;
Pathway	Androgen biosynthesis, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Metabolism of steroid hormones and vitamins A and D, organism-specific biosystem; Steroid hormone biosynthesis, organism-specific biosystem; Steroid hormone biosynthesis, conserved biosystem; androgen biosynthesis, organism-specific biosystem;
MIM	184753

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Background

Case Study

Applications

Q & A

Customer Reviews

The Human SRD5A1 Stable Cell Line, based on the HEK293 cell line, is designed to express the SRD5A1 (Steroid 5-alpha-reductase 1) gene. SRD5A1 is an enzyme involved in the conversion of testosterone to dihydrotestosterone (DHT), which is a more potent androgen. This enzyme plays a crucial role in the development of male reproductive organs and secondary sexual characteristics, as well as in the regulation of hair growth and prostate function. Mutations in SRD5A1 can lead to various disorders, including androgen insensitivity syndrome and benign prostatic hyperplasia. The HEK293 cell line, with its high transfection efficiency and ability to support stable expression, is an ideal host for studying the function of SRD5A1 and its role in health and disease. This stable cell line is valuable for research into the mechanisms of androgen metabolism and for the development of therapies targeting SRD5A1-mediated pathways.

The enzyme steroid 5-α reductase (5AR) is in charge of converting steroids into 5-α reduced metabolites, like 5-α dihydrotestosterone (DHT) from testosterone. To evaluate the inhibition of steroid 5-α reductase (5AR), which is essential for converting steroids like testosterone to 5-α dihydrotestosterone (DHT), the researchers created a cell-based assay. They determined the IC50 values for the 5AR inhibitors finasteride and dutasteride using human and fish cell lines in conjunction with a transient overexpression technique. It's interesting to note that fish cell lines had a lower IC50 for dutasteride than human cell lines, suggesting species-specific responses. This sensitive test exhibits excellent reproducibility and is appropriate for screening endocrine-disrupting chemicals (EDCs). It makes use of chemical derivatization and liquid chromatography-tandem mass spectrometry. Subsequent investigations will delve deeper into the inhibition of 5AR by EDCs in other species to clarify their influence on endocrine pathways.

Figure 1 displays the activity of 5α-reductase in transfected HEK-293 cells and the inhibitory effects of finasteride and dutasteride. (doi: 10.3390/molecules26040893) Figure 1. The researchers used non-vector- and SRD5A1-HEK293 cells and SRD5A2-HEK293 cells to measure DHT levels after testosterone addition. KM values were calculated based on the enzyme's affinity. Inhibition assays with finasteride and dutasteride followed, determining IC50 values. (Kim D, et al., 2021)

The Human SRD5A1 Stable Cell Line - HEK293 is a valuable resource for studying the function of the SRD5A1 gene, which encodes the enzyme steroid 5-alpha-reductase 1. This enzyme is involved in the conversion of testosterone to dihydrotestosterone (DHT), a process that has significant implications for various physiological processes and diseases. The applications of this cell line include:

(1) Androgen Metabolism Research: By expressing SRD5A1 in HEK293 cells, researchers can study the regulation of androgen metabolism, which is critical for understanding male reproductive development, prostate growth, and the development of androgen-dependent diseases such as benign prostatic hyperplasia (BPH) and prostate cancer.

(2) Drug Discovery for Androgen-Related Disorders: The SRD5A1 enzyme is a target for drugs that treat conditions like androgenetic alopecia and hirsutism. This cell line can be used to screen for and identify novel inhibitors or modulators of SRD5A1 activity, potentially leading to new therapeutic agents for these conditions.

(3) Systems Biology and Signaling Pathway Analysis: The HEK293 cell line is known for its versatility in expressing foreign genes. The stable expression of SRD5A1 in this cell line allows for the investigation of the broader signaling pathways affected by androgen metabolism, including those involved in cell growth, differentiation, and apoptosis.

Customer Q&As

How does Human SRD5A1 Stable Cell Line - HEK293 compare with other stable cell lines in terms of SRD5A1 expression stability?

A: Human SRD5A1 Stable Cell Line - HEK293 is specifically engineered for stable SRD5A1 expression in HEK293 cells. Its stability is rigorously tested over long passages to ensure consistent and reliable expression levels.

What methods are employed to validate the authenticity of SRD5A1 expression in Human SRD5A1 Stable Cell Line - HEK293?

A: Authentication of SRD5A1 expression in Human SRD5A1 Stable Cell Line - HEK293 involves techniques such as PCR sequencing, Western blotting, and immunofluorescence staining. These methods confirm the presence and integrity of the SRD5A1 gene and protein.

Can Human SRD5A1 Stable Cell Line - HEK293 be utilized in functional assays related to SRD5A1 activity?

A: Yes, Human SRD5A1 Stable Cell Line - HEK293 is suitable for functional assays assessing SRD5A1 activity, such as enzymatic assays measuring substrate conversion rates. These assays provide valuable insights into the functionality of expressed SRD5A1 protein.

How are potential off-target effects minimized in Human SRD5A1 Stable Cell Line - HEK293?

A: Off-target effects in Human SRD5A1 Stable Cell Line - HEK293 are minimized through careful selection of stable integration sites and thorough screening for single-copy integration. Additionally, control experiments with wild-type cells serve to validate specific effects.

What strategies are employed to enhance the efficiency of SRD5A1 expression in Human SRD5A1 Stable Cell Line - HEK293?

A: Strategies to enhance SRD5A1 expression in Human SRD5A1 Stable Cell Line - HEK293 include optimizing promoter sequences, codon usage, and post-translational modification pathways. These optimizations improve protein yield and stability.

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Customer Reviews

Stable cell line

HEK293 cells are stably engineered to ensure long-term stable expression of SRD5A1.

Germany

2020-04-06

High yield

Human SRD5A1 Stable Cell Line generates a large quantity of SRD5A1, providing ample samples for research.

United States

2020-01-05

Ease of operation

The cell line is easy to culture and handle, providing convenience to researchers.

United Kingdom

2023-10-13

Consistency

Each batch of Human SRD5A1 Stable Cell Line maintains consistent performance and expression levels, ensuring result reproducibility.

Canada

2020-11-19

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Human SRD5A1 Stable Cell Line - HEK293

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Case Study

Applications

Q & A

Customer Reviews

How does Human SRD5A1 Stable Cell Line - HEK293 compare with other stable cell lines in terms of SRD5A1 expression stability?

What methods are employed to validate the authenticity of SRD5A1 expression in Human SRD5A1 Stable Cell Line - HEK293?

Can Human SRD5A1 Stable Cell Line - HEK293 be utilized in functional assays related to SRD5A1 activity?

How are potential off-target effects minimized in Human SRD5A1 Stable Cell Line - HEK293?

What strategies are employed to enhance the efficiency of SRD5A1 expression in Human SRD5A1 Stable Cell Line - HEK293?

Stable cell line

High yield

Ease of operation

Consistency

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