Human PKD2L1 Stable Cell Line - CHO-K1

Name: Human PKD2L1 Stable Cell Line - CHO-K1
SKU: CSC-SC011839-1

Cat.No. : CSC-SC011839-1 Host Cell: CHO-K1

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Cell Line Information

Cell Culture Information

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Cat. No.	CSC-SC011839-1
Description	This cell line is engineered to stably express human polycystin 2 like 1, transient receptor potential cation channel (PKD2L1) in CHO-K1 cells.
Gene	PKD2L1
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Stability	Validated for at least 10 passages
Application	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Shipping	Dry ice
Storage	Liquid nitrogen

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

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Stable human PKD2L1 cell line A cell line called CHO-K1 has been modified to express Polycystin 2 Like 1 (PKD2L1), a protein that is part of the polycystin family. The 10q24.31 chromosome is home to the PKD2L1 gene. Multiple transmembrane domains with cytoplasmic N- and C-termini are present in the encoded protein. In cell-cell and matrix interactions, it performs the role of a nonselective cation channel that is controlled by calcium.
A heterotetrameric, non-selective cation channel that is permeable to calcium has PKD2L1 as one of its pore-forming subunits. In primary cilia, it creates a calcium-permeant ion channel that controls the calcium content in the cilium and sonic hedgehog/SHH signaling. Furthermore, gustatory cells' perception of sour taste is aided by PKD2L1, which is activated by pH fluctuations and low pH. It might also be involved in how water and carbonation taste are perceived by the senses. Chinese hamster ovary (CHO) cells are the source of the CHO-K1 cell line. These adherent cells are extensively utilized in pharmacology and biotechnology for the generation of recombinant proteins and protein expression. If the right culture medium and conditions are used, they can be kept alive in vitro.

PKD2L1 Channel Formation: A heterotetrameric, non-selective cation channel permeable to calcium is created by CHO-K1 cells expressing PKD2L1, and it is necessary for a variety of cell interactions.

Calcium Regulation: SHH signaling and ciliary function are impacted by PKD2L1's control of calcium levels in primary cilia.

Taste Perception: In addition to reacting to pH changes and helping gustatory cells perceive sour taste, PKD2L1 may also be involved in sensing carbonation and water.

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