Cat.No. : CSC-SC011839-1 Host Cell: CHO-K1
| Cat. No. | CSC-SC011839-1 |
| Description | This cell line is engineered to stably express human polycystin 2 like 1, transient receptor potential cation channel (PKD2L1) in CHO-K1 cells. |
| Gene | PKD2L1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The researchers constructed a truncated complex containing the C-terminal TRP fold fragment of PKD1L3, which retained Ca2+ and acid-induced channel activity. Through cryo-electron microscopy, the core heterocomplex structures with 3.1 Å and 3.4 Å resolution were obtained with and without Ca2+ supplementation respectively. The heterotetramer presents a pseudosymmetric TRP structure in a 1:3 ratio, and the selectivity filter (SF) is guarded by Lys2069 of PKD1L3 and Asp523 of the three PKD2L1 subunits. When Ca2+ enters the SF vestibule, Lys2069 of PKD1L3 swings. S6 of PKD1L3 is pushed inward by the S4-S5 chain of adjacent PKD2L1, and the extended intracellular gate closes the pore.
Figure 1. The researchers cloned the cDNA of mouse PKD1L3 and PKD2L1 into pCAG vector respectively, and used HEK293F cells for transient transfection and expression, and then performed protein purification through multi-step affinity purification and size exclusion chromatography. (Su Q, et al., 2021)
Using Creative Biogene's Human PKD2L1 Stable Cell Line - CHO-K1 cell line can simplify the experimental process, improve experimental efficiency and data consistency, and help obtain experimental results faster.
A: Human PKD2L1 Stable Cell Line-CHOK1 has unique advantages in the study of calcium signaling pathways because PKD2L1 is a calcium channel protein involved in the regulation of intracellular calcium ion concentration. This makes this cell line an ideal model for studying calcium signaling pathways and related physiological processes, such as sensing and transducing calcium signals, regulating cell cycle and apoptosis, and functional studies of calcium-dependent enzymes and channels. CHOK1 cells are easy to transfect and culture, further enhancing the flexibility of their experimental applications.
A: Experimentally detecting the functional activity of PKD2L1 can be achieved by calcium imaging technology (such as using calcium indicators Fura2 or Fluo4). These indicators can monitor changes in intracellular calcium ion concentration in real time. Additionally, electrophysiological recordings such as whole-cell patch-clamp techniques can be used to directly measure PKD2L1-mediated current changes. Western Blotting and immunofluorescence staining can be used to verify the expression and localization of PKD2L1 protein.
A: Human PKD2L1 Stable Cell Line-CHOK1 is suitable for screening drugs that modulate calcium signaling pathways. This includes evaluating potential calcium channel inhibitors or activators and studying the effects of drugs on diseases related to the calcium signaling pathway, such as polycystic kidney disease and certain types of heart disease. In addition, this cell line is also suitable for high-throughput screening, using automated calcium imaging and electrophysiology equipment to quickly evaluate the effects of large numbers of compounds, thereby accelerating the development of new drugs.
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The human PKD2L1 stable cell line-CHO-K1 provided by this supplier has good stability, and the cells show consistent growth and stable gene expression during culture. In my observations, I found that these cells can maintain good conditions under appropriate conditions in the cell culture medium, providing reliable support for experiments and research.
For this human PKD2L1 stable cell line - CHO-K1, I feel okay. The stability of the cells is considerable, and gene expression is relatively stable. Although there may be some challenges during the culture process, overall I am pleased with the performance of this cell line.
The human PKD2L1 stable cell line-CHO-K1 provided by this supplier is pretty good. The cells showed a certain stability during the culture process, and gene expression was relatively reliable. During use, I noticed good cell growth and stable experimental results.
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