Human HOXA9 Lentivirus (CMV, Puro)

Name: Human HOXA9 Lentivirus (CMV, Puro)
SKU: LVIM015Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : LVIM015Z

Storage : -80℃ Shipping : Frozen on dry ice

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Virus Particles Information

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Gene Information

Cat. No.	LVIM015Z
Description	Lentivirus expressing untagged Human HOXA9 under the control of CMV promoter, and puromycin resistance marker for mammalian cell selection.
Gene	HOXA9
Species	Human
Applications	Human HOXA9 Lentivirus (CMV, Puro) is a genetic engineering tool that is widely used in the study of cell biology and molecular biology. The following are some of the application areas of Human HOXA9 Lentivirus (CMV, Puro): Cell immortalization: HOXA9 has great application potential in cell immortalization. By using HOXA9 Lentivirus, researchers can efficiently integrate the HOXA9 gene into the target cell genome, thereby inducing these cells to continue to divide and proliferate. This method can be used to establish immortalized cell lines, which has greatly promoted the development of cell biology research, drug screening, and gene function research. Hematological system disease research: HOXA9 is closely related to the normal development and diseases of the blood system (such as leukemia). Using HOXA9 Lentivirus, the specific mechanism of HOXA9 in blood cell differentiation and malignant transformation can be deeply studied. Gene regulation mechanism research: HOXA9 plays an important role in gene regulation, especially gene expression regulation. Through HOXA9 Lentivirus-mediated gene overexpression or knockdown, researchers can analyze how HOXA9 affects the expression of target genes, which helps to better understand gene regulatory networks and their role in different biological processes. Tumor research: Abnormal expression of HOXA9 is associated with the occurrence and development of many types of cancer (such as breast cancer, lung cancer, etc.). Through HOXA9 Lentivirus, its specific functional mechanisms in these cancers can be studied, thereby providing new molecular targets for tumor diagnosis, prognosis and treatment. Drug screening and validation: Establishing a cell model with HOXA9 overexpression or knockdown can be used for high-throughput screening and evaluation of the regulatory effects of new drugs or compounds on HOXA9. This is of great significance for the discovery of new therapeutic drugs.
Titer	Varies lot by lot, for example, ≥110^7 TU/mL, ≥110^8 TU/mL, ≥1*10^9 TU/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma	Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity	Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility	The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation	All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.

Gene Name	HOXA9 homeobox A9 [ Homo sapiens ]
Gene Symbol	HOXA9
Synonyms	HOX1; ABD-B; HOX1G; HOX1.7
Gene Description	homeobox A9
GeneID	3205
Uni ProtID	P31269
mRNA Refseq	NM_152739.3
Protein Refseq	NP_689952.1
Chromosome Location	7p15.2
Function	enzyme binding; protein binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity;
Pathway	TGF-beta Receptor Signaling Pathway, organism-specific biosystem; Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem;
MIM	142956

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Background

Q & A

Customer Reviews

The human HOXA9 lentivirus (CMV, Puro) is a sophisticated genetic tool designed for advanced studies in gene expression, developmental biology, and oncology. This lentiviral vector efficiently transduces and stably integrates the HOXA9 gene into the genome of mammalian cells with the help of its powerful CMV promoter. As a member of the homeobox gene family, HOXA9 plays a crucial role in regulating embryonic development and cell differentiation. Aberrant HOXA9 expression has been associated with various forms of leukemia and other malignancies, making it an important focus of biomedical research.

One of the most notable applications of the human HOXA9 lentivirus is in the field of cell immortalization. Immortalized cells bypass normal cellular senescence and can replicate indefinitely. This is particularly advantageous in research, where long-term studies can be performed without the complications of cellular senescence. HOXA9 is involved in the regulation of genes that control the cell cycle and proliferation. By inducing its expression with a lentiviral vector, researchers can effectively promote indefinite cell divisions, thereby creating immortalized cell lines.

Customer Q&As

How stable are the human HOXA9 lentivirus (CMV, Puro)?

A: Pre-made lentivirus are stable for at least one year if they are keptat-80°Cat all times until used(Lentivirus is shipped in dry-ice package)Repeated freeze- thaw cycles should be avoided since virus titer decreases at5% to 10% per cycle. Re-freeze unused particlesor keep them at4C for re-use within 3-5 days.

Can I use human HOXA9 lentivirus (CMV, Puro) to generate the stable cell line?

A: Yes, you can use these particles to generate a stable cell line.Pre-made lentiviral particles contain different selection markersallowing antibiotic selection following transduction.

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Customer Reviews

Good Purchase

I purchase human HOXA9 lentivirus (CMV, Puro) for immortalization of various hematopoietic cells.A GFP–Puromycin Fusion dual marker was expressed under RSV promoter, which allows to select the transduced cell via Puromycin antibiotic or via cell sorting by GFP fluorescent signal.

United States

2022-11-17

Good Product for Cell Immortalization

The product is well suited for cell immortalization and other applications.

United States

2023-04-19

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Human HOXA9 Lentivirus (CMV, Puro)

Virus Particles Information

Quality Control

Gene Information

Background

Q & A

Customer Reviews

How stable are the human HOXA9 lentivirus (CMV, Puro)?

Can I use human HOXA9 lentivirus (CMV, Puro) to generate the stable cell line?

Good Purchase

Good Product for Cell Immortalization

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