Cat.No. : LVIM015Z
Titer: ≥1*10^7 TU/mL / ≥1*10^8 TU/mL / ≥1*10^9 TU/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | LVIM015Z |
| Description | Lentivirus expressing untagged Human HOXA9 under the control of CMV promoter, and puromycin resistance marker for mammalian cell selection. |
| Target Gene | HOXA9 |
| Species | Human |
| Application | Human HOXA9 Lentivirus (CMV, Puro) is a genetic engineering tool that is widely used in the study of cell biology and molecular biology. The following are some of the application areas of Human HOXA9 Lentivirus (CMV, Puro): Cell immortalization: HOXA9 has great application potential in cell immortalization. By using HOXA9 Lentivirus, researchers can efficiently integrate the HOXA9 gene into the target cell genome, thereby inducing these cells to continue to divide and proliferate. This method can be used to establish immortalized cell lines, which has greatly promoted the development of cell biology research, drug screening, and gene function research. Hematological system disease research: HOXA9 is closely related to the normal development and diseases of the blood system (such as leukemia). Using HOXA9 Lentivirus, the specific mechanism of HOXA9 in blood cell differentiation and malignant transformation can be deeply studied. Gene regulation mechanism research: HOXA9 plays an important role in gene regulation, especially gene expression regulation. Through HOXA9 Lentivirus-mediated gene overexpression or knockdown, researchers can analyze how HOXA9 affects the expression of target genes, which helps to better understand gene regulatory networks and their role in different biological processes. Tumor research: Abnormal expression of HOXA9 is associated with the occurrence and development of many types of cancer (such as breast cancer, lung cancer, etc.). Through HOXA9 Lentivirus, its specific functional mechanisms in these cancers can be studied, thereby providing new molecular targets for tumor diagnosis, prognosis and treatment. Drug screening and validation: Establishing a cell model with HOXA9 overexpression or knockdown can be used for high-throughput screening and evaluation of the regulatory effects of new drugs or compounds on HOXA9. This is of great significance for the discovery of new therapeutic drugs. |
| Titer | Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc. |
| Size | Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. | |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
A: Pre-made lentivirus are stable for at least one year if they are keptat-80°Cat all times until used(Lentivirus is shipped in dry-ice package)Repeated freeze- thaw cycles should be avoided since virus titer decreases at5% to 10% per cycle. Re-freeze unused particlesor keep them at4C for re-use within 3-5 days.
A: Yes, you can use these particles to generate a stable cell line.Pre-made lentiviral particles contain different selection markersallowing antibiotic selection following transduction.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
I purchase human HOXA9 lentivirus (CMV, Puro) for immortalization of various hematopoietic cells.A GFP–Puromycin Fusion dual marker was expressed under RSV promoter, which allows to select the transduced cell via Puromycin antibiotic or via cell sorting by GFP fluorescent signal.
The product is well suited for cell immortalization and other applications.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
