Cat.No. : CSC-RO0614 Host Cell: MC38
| Cat. No. | CSC-RO0614 |
| Description | This cell line is derived from MC38 and is engineered to stably overexpress Human EPCAM. |
| Gene | EPCAM |
| Gene Species | Homo sapiens (Human) |
| Host Cell | MC38 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Cancer immunotherapy has demonstrated significant therapeutic success in recent years. The researchers looked into the effectiveness of VV-EpCAM BiTE, a recombinant vaccinia virus that encodes an EpCAM-targeting bispecific T-cell engager (BiTE), in changing the tumor microenvironment (TME) to boost antitumor immunity across solid tumors. VV-EpCAM BiTE invaded and lysed malignant cells with high efficiency, boosting EpCAM BiTE secretion. This facilitated the interaction between EpCAM-positive tumor cells and CD3ϵ on T cells, activating naive T cells and causing cytokine release (e.g., IFN-γ, IL-2). Intratumoral delivery of VV-EpCAM BiTE significantly increased anticancer efficacy in tumors with high EpCAM expression, which was associated with increased immune cell infiltration, reduced CD8+ T cell exhaustion, and improved T-cell-mediated immune responses in the TME. These findings support VV-EpCAM BiTE's potential as a treatment method that combines bispecific antibody advantages with oncolytic virus capabilities.
Figure 1. The effect of EPCAM Stable Cell Line - MC38 on T-cell activation and cytotoxicity was assessed utilizing secreted EpCAM BiTE. The researchers evaluated T-cell activation markers CD69 and CD25, cytokine secretion (IFN-γ and IL-2), and T-cell cytotoxicity against 4T1, H22, MC38-EpCAM, and MC38 cells. (Wei M, et al., 2022)
A: The development of the Human EPCAM Stable Cell Line - MC38 involved transfecting MC38 cells with a plasmid containing the human EPCAM gene along with a selectable marker gene, typically antibiotic resistance. Following transfection, cells were subjected to selective pressure using the appropriate antibiotic to isolate those cells containing the desired gene. Subsequently, these selected cells were expanded and characterized to establish a stable cell line expressing human EPCAM.
A: EPCAM, or Epithelial Cell Adhesion Molecule, plays a multifaceted role in cellular physiology. It is involved in cell-cell adhesion, signaling, proliferation, migration, and differentiation. In cancer research, EPCAM is of particular interest due to its overexpression in various epithelial cancers, including colorectal, breast, and lung cancers. EPCAM's involvement in tumor growth, metastasis, and resistance to therapy makes it a potential therapeutic target and diagnostic marker in oncology research.
A: The MC38 cell line is derived from a murine colon adenocarcinoma model. It exhibits characteristics typical of colorectal cancer, making it a valuable tool for studying various aspects of cancer biology, including tumor development, progression, and response to treatment. MC38 cells have been extensively used in preclinical cancer research due to their immunogenicity and ability to form tumors in immunocompetent mice, facilitating studies on tumor immunology, immunotherapy, and tumor microenvironment interactions.
A: Functional assays utilized to assess the role of EPCAM in cellular processes include cell proliferation assays, migration assays, adhesion assays, and assays evaluating signaling pathways associated with EPCAM. These assays provide insights into how EPCAM influences key cellular functions such as proliferation, migration, and adhesion, as well as its involvement in intracellular signaling cascades. Additionally, in vivo assays using mouse models may be employed to evaluate EPCAM's role in tumor growth, metastasis, and response to therapy within a physiological context.
A: EPCAM expression in cancer cells can influence various signaling pathways involved in tumor development and progression. One such pathway is the Wnt/β-catenin signaling pathway, where EPCAM interacts with β-catenin to regulate cell proliferation, migration, and stemness properties. Additionally, EPCAM can modulate EGFR (epidermal growth factor receptor) signaling, leading to increased cell survival and proliferation. Furthermore, EPCAM-mediated signaling crosstalk with other pathways such as MAPK/ERK (mitogen-activated protein kinase/extracellular signal-regulated kinase) and PI3K/Akt (phosphatidylinositol-3-kinase/protein kinase B) pathways contributes to tumor growth, invasion, and metastasis.
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The Human EPCAM Stable Cell Line - MC38, through specific genetic engineering, stably expresses the human EPCAM protein, ensuring high specificity and consistent expression in tumor biology research, providing an accurate model for studying the role of EPCAM in tumor development and metastasis.
As EPCAM is an important marker for various cancers, the Human EPCAM Stable Cell Line - MC38 enables researchers to directly study the function of EPCAM in tumor formation and progression, accelerating the identification and validation of tumor markers.
The stable expression of the Human EPCAM Stable Cell Line - MC38 ensures consistency and reproducibility of experimental results, providing a reliable data foundation for tumor-related research.
The Human EPCAM Stable Cell Line - MC38 can be used for high-throughput screening of antibodies or small molecule drugs, especially therapies targeting EPCAM, speeding up new drug discovery and the development of tumor treatment strategies.
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