Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RO0541
Host Cell : HEK293T Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RO0541 |
| Description | This cell line is derived from HEK293T and is engineered to stably overexpress Human EPCAM. |
| Target Gene | EPCAM |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Applications |
1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | EPCAM epithelial cell adhesion molecule [ Homo sapiens ] |
| Gene Symbol | EPCAM |
| Synonyms | ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1 |
| Gene ID | 4072 |
| Uni Prot ID | P16422 |
| m RNA Refseq | BC014785 |
| Chromosome Location | 2p21 |
| Function | protein binding; |
| MIM | 185535 |
Epithelial Cell Adhesion Molecule (EPCAM) is a transmembrane glycoprotein encoded by the EPCAM gene. Its discovery dates back to 1982 by Herlyn et al., marking a significant milestone in cancer research. EPCAM is implicated in cell adhesion, proliferation, and migration processes, playing crucial roles in embryogenesis, tissue homeostasis, and cancer progression. The Human EPCAM Stable Cell Line - HEK293T, established in 1997 by Graham et al., serves as a vital tool in biomedical research. It consists of human embryonic kidney (HEK) cells expressing stable levels of EPCAM, enabling the investigation of EPCAM's function, regulation, and interactions in a controlled cellular environment. This cell line has facilitated numerous studies elucidating EPCAM's involvement in carcinogenesis, metastasis, and therapeutic resistance. Furthermore, it has paved the way for the development of targeted therapies and diagnostic approaches for EPCAM-associated malignancies.
Epithelial cell adhesion molecule (EpCAM) is widely expressed in various epithelial carcinomas and plays crucial roles in cell adhesion and proliferation. Researchers investigated the role of EpCAM in nasopharyngeal carcinoma (NPC) using the EPCAM Stable Cell Line. They observed higher EpCAM expression in NPC samples compared to non-cancerous tissue, with high expression correlating with metastasis and reduced survival. EpCAM overexpression in NPC cells promoted epithelial-mesenchymal transition (EMT) and a cancer stem cell (CSC)-like phenotype, enhancing metastasis via the PTEN/AKT/mTOR pathway. These findings suggest EpCAM as a potential therapeutic target in NPC.
Figure 1. Researchers utilized the EPCAM Stable Cell Line to investigate the role of EpCAM in nasopharyngeal carcinoma (NPC) cells. Lentiviral vectors were employed to stably express or silence EpCAM in NPC cell lines S18, 6–10B, and HONE1. Stable expression and knockdown were confirmed by western blot analysis. This facilitated the study of EpCAM's impact on NPC cell behavior, offering insights into its potential as a therapeutic target in NPC treatment. (Wang MH, et al., 2018)
1. Cell Biology Research: HEK293T cell line engineered with Human EPCAM serves as a model for studying EPCAM-mediated cellular processes, such as cell adhesion and signaling pathways.
2. Cancer Biology Studies: Utilize HEK293T cells expressing Human EPCAM to investigate the role of EPCAM in cancer progression, metastasis, and therapeutic interventions.
3. Drug Screening Assays: Employ HEK293T EPCAM stable cell line to screen for compounds targeting EPCAM-related pathways, aiding in drug discovery for cancer and other diseases.
4. Immunotherapy Development: Evaluate the efficacy of immunotherapeutic agents by analyzing their effects on HEK293T cells expressing Human EPCAM, mimicking tumor microenvironments.
5. Gene Editing Research: Use HEK293T EPCAM stable cell line for CRISPR/Cas9-mediated gene editing studies targeting EPCAM gene, facilitating functional characterization and disease modeling.
A: CHO-K1 cells were likely chosen for their ability to express recombinant proteins efficiently, making them suitable for studying MSLN biology, tumor targeting, and therapeutic antibody development.
A: Stability and expression were likely assessed through methods such as flow cytometry, immunoblotting, or functional assays measuring MSLN-mediated binding or internalization, with continuous selection pressure applied.
A: Characterization may involve analysis of MSLN membrane localization, binding affinity to targeting ligands or antibodies, downstream signaling pathways, and functional implications in tumor cell proliferation, migration, or therapeutic resistance.
A: Quality control likely included confirmation of MSLN expression levels, validation of its binding specificity and functional activity, assessment of off-target effects, and validation of phenotypic changes associated with MSLN modulation.
A: Comparative analysis with primary tumor cells or in vivo models helps validate the relevance of MSLN expression in tumor progression, metastasis, and its potential as a target for antibody-based cancer therapies, guiding the development of MSLN-targeted therapeutics.
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Outstanding stability! The Human EPCAM Stable Cell Line in HEK293T cells delivers consistent EPCAM expression, ensuring robust results in my cancer and stem cell research.
Facilitating advanced studies! With stable EPCAM expression, I can investigate cancer stem cell biology and epithelial cell signaling pathways with confidence, advancing my research goals.
Impressive reliability! This cell line surpasses expectations, providing a reliable platform for studying EPCAM-targeted therapies and cancer immunotherapy.
Enhancing research efficiency! Its stable expression streamlines experimental workflows, simplifying data analysis and accelerating scientific discoveries.
An indispensable resource! The Human EPCAM Stable Cell Line has significantly contributed to my research, offering valuable insights into EPCAM-mediated tumor progression and potential therapeutic interventions.
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