Cat.No. : CSC-RO0541 Host Cell: HEK293T
| Cat. No. | CSC-RO0541 |
| Description | This cell line is derived from HEK293T and is engineered to stably overexpress Human EPCAM. |
| Gene | EPCAM |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Epithelial cell adhesion molecule (EpCAM) is widely expressed in various epithelial carcinomas and plays crucial roles in cell adhesion and proliferation. Researchers investigated the role of EpCAM in nasopharyngeal carcinoma (NPC) using the EPCAM Stable Cell Line. They observed higher EpCAM expression in NPC samples compared to non-cancerous tissue, with high expression correlating with metastasis and reduced survival. EpCAM overexpression in NPC cells promoted epithelial-mesenchymal transition (EMT) and a cancer stem cell (CSC)-like phenotype, enhancing metastasis via the PTEN/AKT/mTOR pathway. These findings suggest EpCAM as a potential therapeutic target in NPC.
Figure 1. Researchers utilized the EPCAM Stable Cell Line to investigate the role of EpCAM in nasopharyngeal carcinoma (NPC) cells. Lentiviral vectors were employed to stably express or silence EpCAM in NPC cell lines S18, 6–10B, and HONE1. Stable expression and knockdown were confirmed by western blot analysis. This facilitated the study of EpCAM's impact on NPC cell behavior, offering insights into its potential as a therapeutic target in NPC treatment. (Wang MH, et al., 2018)
A: CHO-K1 cells were likely chosen for their ability to express recombinant proteins efficiently, making them suitable for studying MSLN biology, tumor targeting, and therapeutic antibody development.
A: Stability and expression were likely assessed through methods such as flow cytometry, immunoblotting, or functional assays measuring MSLN-mediated binding or internalization, with continuous selection pressure applied.
A: Characterization may involve analysis of MSLN membrane localization, binding affinity to targeting ligands or antibodies, downstream signaling pathways, and functional implications in tumor cell proliferation, migration, or therapeutic resistance.
A: Quality control likely included confirmation of MSLN expression levels, validation of its binding specificity and functional activity, assessment of off-target effects, and validation of phenotypic changes associated with MSLN modulation.
A: Comparative analysis with primary tumor cells or in vivo models helps validate the relevance of MSLN expression in tumor progression, metastasis, and its potential as a target for antibody-based cancer therapies, guiding the development of MSLN-targeted therapeutics.
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Outstanding stability! The Human EPCAM Stable Cell Line in HEK293T cells delivers consistent EPCAM expression, ensuring robust results in my cancer and stem cell research.
Facilitating advanced studies! With stable EPCAM expression, I can investigate cancer stem cell biology and epithelial cell signaling pathways with confidence, advancing my research goals.
Impressive reliability! This cell line surpasses expectations, providing a reliable platform for studying EPCAM-targeted therapies and cancer immunotherapy.
Enhancing research efficiency! Its stable expression streamlines experimental workflows, simplifying data analysis and accelerating scientific discoveries.
An indispensable resource! The Human EPCAM Stable Cell Line has significantly contributed to my research, offering valuable insights into EPCAM-mediated tumor progression and potential therapeutic interventions.
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