CAG-Cre-mCherry Lentivirus

The genus lentivirus belongs to the retroviridae family and is a diploid RNA virus. The primary infected cells are mainly lymphocytes and macrophages, which cause the infected individuals to become ill. The main clinical feature of lentivirus infection is that it undergoes a long incubation period before the typical clinical symptoms appear, and then the disease slowly develops, so it is called lentivirus. The lentiviral vector is based on the genome of the lentivirus, and several sequence structures related to viral activity are removed to make it biologically safe. Then, the sequence and expression structure of the target gene required for the experiment are introduced into this genome skeleton, and it is prepared into a vector. Early lentiviral vectors were modified from human immunodeficiency virus (HIV), including HIV-1 vector system and HIV-2 vector system. Later, as the research deepened, researchers also developed many other types of lentiviral vector systems, including simian immunodeficiency virus (SIV) vector system, felines immunodeficiency virus (FIV) vector system, equine infectious anemia virus (EIAV) vector system, caprine arthritis-encephalitis virus (CAEV) vector system, bovine immunodeficiency virus (BIV) vector system, sheep myelin shedding virus (visna/maedi virus, VMV) vector system, etc. Obviously, vector systems from different species may be more effective in gene transfection experiments in cells of the corresponding species, and whether lentiviral vectors can be cross-used in cells of different species, especially whether lentiviral systems of other species can be used in humans, requires further research and evaluation.