Hotmaster Taq DNA Polymerase

EMDTS0014

Hotmaster Taq DNA Polymerase is a superior alternative for performing PCRexperiments generally known as "hot start" PCR. Hotmaster blocks the substratebinding site of DNA polymerases in a temperature-dependent manner. Inactivepolymerase-inhibitor complexes are formed at temperatures < 40°c,="" where="" the="" affinity="" of="" hotmaster="" for="" taq="" polymerase="" is="" higher="" than="" the="" binding="" affinity="" of="" the="" template="" dna.="" between="" 40°c="" and="" 55°c="" the="" hotmaster="" competes="" with="" the="" template="" dna="" for="" binding="" to="" the="" taq="" polymerase,="" thereby="" shifting="" the="" binding="" equilibrium="" towards="" complex="" formation="" with="" only="" target-specific="" primed="" template="" dna.="" at="" temperatures="" above="" 55°c="" the="" hotmaster="" inhibitor="" is="" displaced="" from="" complexes="" with="" the="" taq="" polymerase="" by="" target-specific="" primed="" template="" dna.="" a="" unique="" performance="" feature="" of="" the="" hotmaster="" inhibitor="" is="" that="" it="" can="" go="" through="" multiple="" temperature="" cycles="" of="" binding-equilibrium="" competition-dissociation="" during="" pcr="" without="" irreversible="" heat="" inactivation.="" where="" other="" taq="" polymerase="" formulations="" for="" "hot="" start"="" pcr="" block="" the="" activity="" of="" taq="" polymerase="" only="" prior="" to="" the="" first="" high="" temperature="" step,="" the="" creative="" biogene="" hotmaster="" provides="" sustained="" temperature="" control="" throughout="">

For Research Use Only. Not for use in diagnostic procedures.

The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases

250U, 500U

Store at -20°C.

One unit is defined as the amount of the enzyme required to catalyze theincorporation of 10 nmoles of dNT P's into an acid-insoluble form in 30 minutes at74°C using hering sperm DNA as substrate.