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Cat. No. : EMDTS0015
| Cat. No. | EMDTS0015 |
| Description | Creative Biogene Long Taq DNA Polymerase is a mix of Taq DNA polymerase anda powerful high-fidelity enzyme to ensure high amplification efficiency andmaximum fidelity. Long Taq DNA Polymerase has a 3'→5' Exonuclease Activity.Two different unique PCR buffers are suitable for different templates andenhance extension rates and fidelity, even when amplifying complex templates.PCR products of up to 40 kb can be reliably amplified. |
| Size | 250U, 500U |
| Unit Definition | One unit is defined as the amount of the enzyme required to catalyze theincorporation of 10 nmoles of dNT P's into an acid-insoluble form in 30 minutes at74°C using hering sperm DNA as substrate. |
| Quality Control | The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleasesconfirmed by appropriate quality tests. Functionally tested in PCR. |
| Note | For Research Use Only. Not for use in diagnostic procedures. |
| Storage | Store at -20°C. |
A: This enzyme is a mixture of pfu DNA Polymerase and Taq DNA polymerase in a specific ratio, to ensure high amplification efficiency and maximum fidelity.
A: No, it has two different unique PCR buffer solutions, suitable for different templates, and need to be selected according to the instruction manual.
A: Yes, this enzyme has about 5-fold PCR reliability of Taq DNA polymerase, it can reliably amplify PCR products up to 40 kb.
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The enzyme has 2 buffer systems to improve elongation and fidelity even when amplifying complex templates.
The enzyme can polymerize at a speed of 10-15 seconds/kb, which can save researchers up to 70% of their experimental time.
The molecular weight of this enzyme is smaller than the normal Taq enzyme, and it has higher enzyme activity with the same amount of enzyme, and it can significantly reduce the non-specific bands and primer dimerization.
The T1/2 of Long-Taq enzyme=2.5-3 hour(100℃),12 hour(95℃),T1/2 of normal Taq enzyme=1.6 hour(95℃),which is suitable for amplification of high GC content template.
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