T7 DNA Polymerase

Name: T7 DNA Polymerase
SKU: EMQZ1415

For research use only. Not intended for any clinical use.

Cat.No.

EMQZ1415

Description

T7 DNA Polymerase, a template dependent DNA polymerase, catalyzes DNA synthesis in the 5' to 3' direction. It is a highly processive DNA polymerase allowing continuous synthesis of long stretches of DNA. The enzyme also exhibits a high 3' to 5' exonuclease activity towards single- and double-stranded DNA.

Applications

• Purification of covalently closed circular DNA by removal of residual genomic DNA• Primer extension reactions on long templates• DNA 3'-end labeling• Strand extensions in site-directed mutagenesis• Fill-in blunting of 5'-overhang DNA• Second strand synthesis of cDNA• In situ detection of DNA fragmentation associated with apoptosis

Concentration

10,000 U/ml

Size/Form

1,000 U; 5,000 U

Source

E. coli strain

Storage

Store at -20°C

Publications

Q & A

Customer Reviews

Customer Q&As

What are the advantages of T7 DNA Polymerase?

A: The T7 DNA Polymerase has a strong 3'→5' exonuclease activity, approximately 1000 times greater than Klenow fragment, and it is active in restriction endonuclease buffers.

Do the assays for the T7 DNA Polymerase require long incubation times?

A: No, the determination of T7 DNA Polymerase does not require long incubation times at 37°C.

How can T7 DNA Polymerase be applied?

A: The T7 DNA Polymerase can be used for the purification of covalently closed circular DNA by removing residual genomic DNA and also for the in situ detection of DNA fragments associated with apoptosis.

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