phi29 DNA Polymerase

Name: phi29 DNA Polymerase
SKU: EMQZ1419

For research use only. Not intended for any clinical use.

Cat.No.

EMQZ1419

Description

phi29 DNA Polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 (Φ29). This polymerase has exceptional strand displacement and processive synthesis properties. The polymerase has an inherent 3´→5' proofreading exonuclease activity.

Applications

• Replication requiring a high degree of strand displacement and/or processive synthesis• High fidelity replication at moderate temperatures

Concentration

10,000 U/ml

Reaction Conditions

1× phi29 DNA Polymerase Reaction Buffer, 200 ug/ml BSA,incubate at 30°C.

Size/Form

250 U; 1,250 U

Source

An E. coli strain that carries the phi29 DNA Polymerase gene from bacteriophage phi29.

Storage

Store at -20°C

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 0.5 pmol of dNTP into acid-insoluble material in 10 minutes at 30°C.

Publications

Q & A

Customer Reviews

Customer Q&As

Does the phi29 DNA Polymerase require thermal cycling for the reaction?

A: No, phi29 DNA polymerase has DNA strand-displacement activity and strong DNA continuous synthesis capability, making it suitable for isothermal DNA amplification in vitro independent of thermal cycling.

Is it suitable for whole-genome amplification?

A: Phi29 DNA polymerase has strong strand affinity, and a single polymerization reaction can achieve continuous extension of over 70 kb in length, making it suitable for stable amplification of the entire genome.

Can phi29 DNA Polymerase amplify linear or circular double-stranded DNA?

A: Yes, however, phi29 DNA polymerase tends to amplify single-stranded DNA or single-stranded RNA substrates, although it can amplify linear or circular double-stranded DNA.

What experiments is ph29 DNA Polymerase suitable for?

A: Phi29 DNA polymerase can be applied to protein-primed DNA amplification, RNA-primed DNA amplification, and cloning in cell-free systems of lethal genes, and other related experiments.

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