phi29 DNA Polymerase

Name: phi29 DNA Polymerase
SKU: EMQZ1419
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMQZ1419

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Product Information

Cat. No.	EMQZ1419
Description	phi29 DNA Polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 (Φ29). This polymerase has exceptional strand displacement and processive synthesis properties. The polymerase has an inherent 3´→5' proofreading exonuclease activity.
Source	An E. coli strain that carries the phi29 DNA Polymerase gene from bacteriophage phi29.
Concentration	10,000 U/ml
Applications	• Replication requiring a high degree of strand displacement and/or processive synthesis • High fidelity replication at moderate temperatures
Size	250 U; 1,250 U
Unit Definition	One unit is defined as the amount of enzyme that will incorporate 0.5 pmol of dNTP into acid-insoluble material in 10 minutes at 30°C.
Reaction Conditions	1× phi29 DNA Polymerase Reaction Buffer, 200 ug/ml BSA,incubate at 30°C.
Storage	Store at -20°C

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Customer Q&As

Does the phi29 DNA Polymerase require thermal cycling for the reaction?

A: No, phi29 DNA polymerase has DNA strand-displacement activity and strong DNA continuous synthesis capability, making it suitable for isothermal DNA amplification in vitro independent of thermal cycling.

Is it suitable for whole-genome amplification?

A: Phi29 DNA polymerase has strong strand affinity, and a single polymerization reaction can achieve continuous extension of over 70 kb in length, making it suitable for stable amplification of the entire genome.

Can phi29 DNA Polymerase amplify linear or circular double-stranded DNA?

A: Yes, however, phi29 DNA polymerase tends to amplify single-stranded DNA or single-stranded RNA substrates, although it can amplify linear or circular double-stranded DNA.

What experiments is ph29 DNA Polymerase suitable for?

A: Phi29 DNA polymerase can be applied to protein-primed DNA amplification, RNA-primed DNA amplification, and cloning in cell-free systems of lethal genes, and other related experiments.

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