Taq DNA Polymerase (for PAGE)

Name: Taq DNA Polymerase (for PAGE)
SKU: EMDTS0010
Rating: 4.0 (2 reviews)

For research use only. Not intended for any clinical use.

Cat.No.

EMDTS0010

Description

Taq DNA Polymerase is a recombinant 94 kDa DNA polymerase originally isolated from Thermus aquaticus, and expressed in E. coli. This enzyme has a 5′→3′ DNA polymerase and a 5′→3′ exonuclease activity but lacks a 3′→5′ exonuclease activity. Taq DNA polymerase catalyzes the non-template directed addition of an adenine residue to the 3′-end of both strands of DNA molecules to make it suitable for TA cloning.

Applications

PCR; RT-PCR; Differential display; Multiplex PCR;DNA sequencing; PAGE

Features

`High amplification efficiency`Reproducible results

Note

For Research Use Only. Not for use in diagnostic procedures.

Size/Form

500U, 2000U

Storage

Store at -20℃

Publications

Q & A

Customer Reviews

Customer Q&As

Are there any concerns about using the enzyme to amplify samples that may be contaminated and affect recovery?

A: No, the enzyme does not contain other nucleases, exonucleases, or bacterial DNA.

Will it be affected if the enzyme is accidentally left at room temperature overnight?

A: Generally, if stored at room temperature for a short period of time (within a week), its activity will not change significantly.

Can the fragments amplified by this enzyme be ligated to a T vector?

A: Yes, it can, its PCR products have a 3' end with A, which can be cloned into a T vector.

Can it be used for clinical diagnosis?

A: No, it can only be used for research.

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Customer Reviews

High fidelity and high specificity.

This enzyme has both 5'→3' DNA polymerase and 5'→3' exonuclease activity, which improves the fidelity.

United States

01/13/2023

Inexpensive and efficient

It is inexpensive and efficient for amplification and the results have high reproducibility.

United States

01/23/2023

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Taq DNA Polymerase (for PAGE)

Publications

Q & A

Customer Reviews

Are there any concerns about using the enzyme to amplify samples that may be contaminated and affect recovery?

Will it be affected if the enzyme is accidentally left at room temperature overnight?

Can the fragments amplified by this enzyme be ligated to a T vector?

Can it be used for clinical diagnosis?

High fidelity and high specificity.

Inexpensive and efficient

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