Panoply™ Human PSCA Knockdown Stable Cell Line

Prostate stem cell antigen (PSCA) is associated with cancer disease progression, angiogenesis, invasion, metastasis, and immune evasion. However, its expression patterns and diagnostic and prognostic potential have not been thoroughly analyzed from a pan-cancer perspective. Here, researchers aimed to investigate the impact of PSCA on prognosis and inflammatory cell infiltration patterns in different cancer types. They analyzed the relationship between PSCA expression and immune subtypes within the tumor microenvironment (TME), as well as the role of molecular subtypes, potentially promising immune biomarkers, and tumor-infiltrating lymphocytes (TILs) in various cancer types, specifically lung adenocarcinoma (LUAD). The co-expression network of PSCA was found to be primarily involved in regulating immune responses, as well as antigen processing and expression, and was significantly enriched in pathways related to cancer pathology and metabolism. Together, these studies suggest that PSCA is a promising target for immunotherapy in cancer patients.

Western blotting and qRT-PCR assays showed that PSCA expression was higher in lung cancer tissues than in adjacent adjacent tissues (Figures 1A and B). Subsequently, PSCA expression was examined in four lung cancer cell lines. High PSCA expression was observed in H1299 and A549 cells (Figures 1C and D). Three siRNAs were used to knock down PSCA in H1299 and A549 cells, with si-PSCA-1 showing the most significant effect (Figures 1E and F). In PSCA-knockdown H1299 and A549 cells, cell migration and invasion were reduced (Figures 1G and H), while apoptosis was promoted (Figures 1I and K). Furthermore, PSCA knockdown induced lung cancer cells to exit the cell cycle (Figures 1J and L).

Figure 1. PSCA expression is upregulated in tumour tissues and promotes tumour cell migration, invasion and proliferation. (Wang C, et al., 2024)