Panoply™ Human EPHB4 Knockdown Stable Cell Line

Name: Panoply™ Human EPHB4 Knockdown Stable Cell Line
SKU: CSC-DC004995
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-DC004995

Host Cell : HEK293 (Hela and other cell types are also available) Validation : Real-Time RCR

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Gene Information

Cat. No.	CSC-DC004995
Description	Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene	EPHB4
Host Cell	HEK293 (Hela and other cell types are also available)
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid Nitrogen
Shipping	Dry Ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	EPHB4 EPH receptor B4 [ Homo sapiens ]
Gene Symbol	EPHB4
Synonyms	EPHB4; EPH receptor B4; EphB4 , HTK; ephrin type-B receptor 4; Tyro11; ephrin receptor EphB4; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; hepatoma transmembrane kinase; tyrosine-protein kinase TYRO11; tyrosine-protein kinase receptor HTK; HTK; MYK1; TYRO11;
GeneID	2050
Uni ProtID	P54760
mRNA Refseq	BC052804
Chromosome Location	7q22
Function	ATP binding; ephrin receptor activity; ephrin receptor activity; nucleotide binding; protein binding; protein tyrosine kinase activity; receptor activity; transmembrane receptor protein tyrosine kinase activity;
Pathway	Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; EPHB forward signaling, organism-specific biosystem; Ephrin B reverse signaling, organism-specific biosystem; EphrinB-EPHB pathway, organism-specific biosystem;
MIM	600011

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Erythropoietin producing human hepatoma (EphB4) promotes the survival, migration, invasion, and angiogenesis of tumor cells from various sources. Here, researchers investigated the effects of EphB4 on the oncogenic potential of renal cell carcinoma cells, including in vitro and in vivo experiments. They used CCK-8 and Annexin V-FITC/PI staining methods to detect the effects of EphB4 knockdown on cell proliferation and cell death. The Boyden chamber assay and wound healing assay were used to detect the effects of EphB4 knockdown on cell invasion and migration. To explore the downstream effects of EphB4 knockdown, the researchers examined the activities of ERK and STAT3. The results showed that EphB4 downregulation inhibited the growth, invasion, and migration of renal cell carcinoma cells and promoted apoptosis. EphB4 knockdown also reduced the levels of p-ERK and p-STAT3. In vivo experiments showed that EphB4 knockdown significantly inhibited the growth of xenograft tumors and reduced the levels of p-ERK and p-STAT3. Therefore, EphB4 knockdown can effectively inhibit the proliferation of human renal cell carcinoma cells and induce their apoptosis both in vitro and in vivo.

Here, researchers overexpressed EphB4, resulting in increased RCC cell proliferation (detected by CCK-8 assay) (Figure 1A-D). Similarly, in EphB4 knockdown cells, cell proliferation was significantly reduced (Figure 1A-D). These results indicate that EphB4 positively regulates cell proliferation. Researchers used Annexin V-FITC/PI double staining to detect the apoptotic effect of EphB4 on RCC cells. Flow cytometry analysis was performed immediately after staining. The results showed that EphB4 downregulation promoted apoptosis. Annexin V-FITC staining revealed early apoptosis in EphB4 knockdown 786-O cells (Figure 1E, 1F). Compared to the control group, the apoptosis rate in EphB4 knockdown cells was significantly increased.

Figure 1. Effect of EphB4 on proliferation and apoptosis of RCC cells. (Zhang Y, et al., 2019)

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