Cat.No. : CSC-RO0046 Host Cell: CHO-K1
| Cat. No. | CSC-RO0046 |
| Description | This cell line is a stably transfected cell line which expresses Human TNFRSF4 without any tag. |
| Background | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation. |
| Gene | TNFRSF4 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | TNFRSF4 tumor necrosis factor receptor superfamily, member 4 [ Homo sapiens ] |
| Gene Symbol | TNFRSF4 |
| Synonyms | OX40; ACT35; CD134; TXGP1L |
| Gene ID | 7293 |
| UniProt ID | P43489 |
| mRNA Refseq | NM_003327.3 |
| Protein Refseq | NP_003318.1 |
| Chromosome Location | 1p36 |
| Function | tumor necrosis factor-activated receptor activity; |
| Pathway | Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem; |
| MIM | 600315 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: When optimizing growth conditions for the Human TNFRSF4 Stable Cell Line-CHO, it's crucial to control factors such as temperature, pH, and nutrient availability. The cells typically thrive at 37°C with a pH of around 7.2-7.4, and require a rich medium like F12K or CDM4CHO. Additionally, the cells may benefit from the presence of animal serum, especially during the initial stages of culture establishment.
A: The Human TNFRSF4 Stable Cell Line-CHO exhibits stress responses similar to other CHO cell lines, including the activation of heat shock proteins and antioxidant defense mechanisms. However, the specific response to stressors may be influenced by the stable expression of TNFRSF4, which could alter cellular signaling pathways involved in stress response.
A: This cell line can be used for high-throughput screening of compounds that modulate TNFRSF4 activity, which is relevant for diseases such as autoimmune disorders and cancer. Additionally, it can serve as a model system to study the effects of drugs on TNFRSF4 signaling pathways.
A: By stably expressing TNFRSF4, this cell line provides a controlled environment to study the signaling cascades initiated by TNFRSF4 ligands. This can lead to insights into the regulation of immune responses and the development of therapeutic strategies targeting TNFRSF4.
A: CHO cells are known for their ability to perform complex glycosylation, which can be critical for the function of secreted proteins. The Human TNFRSF4 Stable Cell Line-CHO may exhibit unique glycosylation patterns due to the presence of TNFRSF4, which could affect the protein's stability, folding, and immunogenicity.
A: Functional validation typically involves assays that measure TNFRSF4-dependent signaling events, such as the activation of downstream kinases or the induction of apoptosis. Reporter gene assays, ELISAs for cytokine release, and flow cytometry to assess receptor expression are common methods used for validation.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
