Human OPRD1-tGFP Stable Cell Line-U2OS

Name: Human OPRD1-tGFP Stable Cell Line-U2OS
SKU: CSC-RG0033
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0033

Host Cell : U2OS Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0033
Target Gene	OPRD1
Gene Species	Homo sapiens (Human)
Abbr	U2OS-HuOPRD1-tGFP
Alias	OPRD1, DOR-1, D-OR-1, OPRD
Host Cell	U2OS
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	OPRD1 opioid receptor, delta 1 [ Homo sapiens ]
Gene Symbol	OPRD1
Synonyms	OPRD1; opioid receptor, delta 1; delta-type opioid receptor; DOR-1; D-OR-1; delta opioid receptor 1; OPRD;
GeneID	4985
Uni ProtID	P41143
Chromosome Location	1p36.1-p34.3
Function	G-protein coupled receptor activity; enkephalin receptor activity; protein binding; receptor activity; signal transducer activity;
Pathway	Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem;
MIM	165195

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Background

Q & A

Customer Reviews

The delta-opioid receptor, also known as delta opioid receptor or simply delta receptor, abbreviated DOR, is an opioid receptor that has enkephalins as its endogenous ligands. Opioid receptors mediate the analgesic action and addictive properties of opioid drugs. The delta-receptor is a promising target for treatment of both drug addiction and mood-related disorders. DOR is also a good target related with Alzheimer′s Disease (AD). Agonist-induced activation of the delta-opioid receptor has been shown to augment beta - and gamma-secretase activities, which increased the production of beta -amyloid peptide (Abeta ), known to accumulate in the brain tissues of Alzheimer"s disease (AD) patients.

Customer Q&As

What is the origin of the U2OS cell line and why was it chosen as the host for creating the Human OPRD1-tGFP Stable Cell Line?

A: The U2OS cell line is derived from human osteosarcoma and is commonly used in research due to its ease of culture and transfection. It was chosen as the host for the Human OPRD1-tGFP Stable Cell Line because it provides a stable and well-characterized background for studying gene expression and protein localization.

What specific techniques or methods were used to create the stable Human OPRD1-tGFP Cell Line-U2OS?

A: To create the stable Human OPRD1-tGFP Cell Line-U2OS, researchers typically employ techniques such as transfection with a plasmid containing the OPRD1 gene fused to a tGFP (turbo green fluorescent protein) reporter gene, followed by selection and maintenance in a suitable culture medium containing antibiotics for stable integration.

What are the advantages of using tGFP as a reporter in the Human OPRD1-tGFP Stable Cell Line-U2OS?

A: The use of tGFP as a reporter in the Human OPRD1-tGFP Stable Cell Line allows for the visualization and tracking of the delta-opioid receptor in live cells. Its bright and stable fluorescence makes it a valuable tool for studying protein localization and dynamics.

How is the expression of the delta-opioid receptor controlled in the Human OPRD1-tGFP Stable Cell Line-U2OS?

A: The expression of the delta-opioid receptor in the Human OPRD1-tGFP Stable Cell Line-U2OS is typically controlled by a strong and constitutive promoter that drives the expression of the OPRD1-tGFP fusion protein. This ensures continuous and stable expression for research purposes.

How can the Human OPRD1-tGFP Stable Cell Line-U2OS contribute to the understanding of opioid receptor biology and potential drug development?

A: This cell line is a valuable tool for studying opioid receptor biology by allowing researchers to investigate receptor localization, trafficking, and interactions with ligands or potential drugs. It can provide insights into receptor function and contribute to the development of novel therapeutic agents targeting opioid receptors.

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