Cat.No. : CSC-RG0033 Host Cell: U2OS
| Cat. No. | CSC-RG0033 |
| Gene | OPRD1 |
| Gene Species | Homo sapiens (Human) |
| Alias | OPRD1, DOR-1, D-OR-1, OPRD |
| Host Cell | U2OS |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The U2OS cell line is derived from human osteosarcoma and is commonly used in research due to its ease of culture and transfection. It was chosen as the host for the Human OPRD1-tGFP Stable Cell Line because it provides a stable and well-characterized background for studying gene expression and protein localization.
A: To create the stable Human OPRD1-tGFP Cell Line-U2OS, researchers typically employ techniques such as transfection with a plasmid containing the OPRD1 gene fused to a tGFP (turbo green fluorescent protein) reporter gene, followed by selection and maintenance in a suitable culture medium containing antibiotics for stable integration.
A: The use of tGFP as a reporter in the Human OPRD1-tGFP Stable Cell Line allows for the visualization and tracking of the delta-opioid receptor in live cells. Its bright and stable fluorescence makes it a valuable tool for studying protein localization and dynamics.
A: The expression of the delta-opioid receptor in the Human OPRD1-tGFP Stable Cell Line-U2OS is typically controlled by a strong and constitutive promoter that drives the expression of the OPRD1-tGFP fusion protein. This ensures continuous and stable expression for research purposes.
A: This cell line is a valuable tool for studying opioid receptor biology by allowing researchers to investigate receptor localization, trafficking, and interactions with ligands or potential drugs. It can provide insights into receptor function and contribute to the development of novel therapeutic agents targeting opioid receptors.
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