Cat.No. : CSC-RG0028 Host Cell: CHO-K1
| Cat. No. | CSC-RG0028 |
| Background | Bradykinin receptor B1. BDKRB1 gene encodes a protein that is one of two Bradykinin receptors. The bradykinin receptor family is a group of G-protein coupled receptors whose principal ligand is the protein bradykinin. This gene encodes a receptor for bradykinin B1. The 9 aa bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation. This receptor associates with G proteins that stimulate a phosphatidylinositol-calcium second messenger system. |
| Gene | BDKRB1 |
| Gene Species | Homo sapiens (Human) |
| Alias | BDKRB1, B1BKR, BKR1, B1R, BKB1R |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human BDKRB1-tGFP Stable Cell Line-CHO is a unique cell line engineered by stably integrating the human BDNF receptor (BDNFR, also known as TrkB) fused with a green fluorescent protein (GFP) into Chinese Hamster Ovary (CHO) cells. This cell line is valuable for neuroscience research because it allows for the visualization of BDNFR activation and downstream signaling pathways in response to BDNF stimulation. The GFP tag enables researchers to monitor receptor localization and trafficking in real-time, providing insights into receptor function and potential therapeutic targets for neurodegenerative diseases.
A: Unlike other cell lines that may express BDNFR, the Human BDKRB1-tGFP Stable Cell Line-CHO offers the advantage of a fluorescent tag, which allows for direct visualization of receptor activity. This feature is particularly beneficial for high-throughput screening of compounds that modulate BDNFR function, as it enables rapid and quantitative assessment of receptor activation without the need for additional labeling steps.
A: This cell line can be used for the development of new therapeutics targeting the BDNFR pathway. By screening libraries of compounds for their ability to modulate receptor activity, researchers can identify potential drug candidates that could enhance or inhibit BDNFR signaling, which is implicated in conditions such as Alzheimer's disease, Parkinson's disease, and depression.
A: The ability to visualize BDNFR activation in real-time using the GFP tag allows researchers to study the dynamics of receptor activity during synaptic plasticity events. This can help elucidate the precise timing and spatial distribution of BDNFR activation, which is crucial for understanding its role in learning and memory processes.
A: The GFP tag on the BDNFR allows for the co-localization and interaction studies with other cellular proteins. Researchers can use this cell line to investigate the formation of protein complexes involving BDNFR, which can provide insights into the regulation of BDNFR signaling and its role in cellular processes.
A: While the cell line provides a simplified model for studying BDNFR, it lacks the complexity of the human brain, including the presence of multiple cell types, the intricate neural network, and the influence of the blood-brain barrier. Therefore, findings from this cell line must be interpreted with caution and validated in more complex models or in vivo.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
