Human BDKRB1-tGFP Stable Cell Line-CHO-K1

Name: Human BDKRB1-tGFP Stable Cell Line-CHO-K1
SKU: CSC-RG0028
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0028

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0028
Background	Bradykinin receptor B1. BDKRB1 gene encodes a protein that is one of two Bradykinin receptors. The bradykinin receptor family is a group of G-protein coupled receptors whose principal ligand is the protein bradykinin. This gene encodes a receptor for bradykinin B1. The 9 aa bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation. This receptor associates with G proteins that stimulate a phosphatidylinositol-calcium second messenger system.
Target Gene	BDKRB1
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuBDKRB1-tGFP
Alias	BDKRB1, B1BKR, BKR1, B1R, BKB1R
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Homo sapiens bradykinin receptor B1 DNA.
Gene Symbol	BDKRB1
Synonyms	B1R; BKR1; B1BKR; BKB1R; BRADYB1; BDKRB1
mRNA Refseq	BC034705

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Customer Reviews

Customer Q&As

What are the specific characteristics of the Human BDKRB1-tGFP Stable Cell Line-CHO that make it a valuable tool for neuroscience research?

A: The Human BDKRB1-tGFP Stable Cell Line-CHO is a unique cell line engineered by stably integrating the human BDNF receptor (BDNFR, also known as TrkB) fused with a green fluorescent protein (GFP) into Chinese Hamster Ovary (CHO) cells. This cell line is valuable for neuroscience research because it allows for the visualization of BDNFR activation and downstream signaling pathways in response to BDNF stimulation. The GFP tag enables researchers to monitor receptor localization and trafficking in real-time, providing insights into receptor function and potential therapeutic targets for neurodegenerative diseases.

How does the Human BDKRB1-tGFP Stable Cell Line-CHO differ from other BDNFR-expressing cell lines in terms of its utility for studying neurotrophin signaling?

A: Unlike other cell lines that may express BDNFR, the Human BDKRB1-tGFP Stable Cell Line-CHO offers the advantage of a fluorescent tag, which allows for direct visualization of receptor activity. This feature is particularly beneficial for high-throughput screening of compounds that modulate BDNFR function, as it enables rapid and quantitative assessment of receptor activation without the need for additional labeling steps.

What are the potential applications of the Human BDKRB1-tGFP Stable Cell Line-CHO in drug discovery for neurological disorders?

A: This cell line can be used for the development of new therapeutics targeting the BDNFR pathway. By screening libraries of compounds for their ability to modulate receptor activity, researchers can identify potential drug candidates that could enhance or inhibit BDNFR signaling, which is implicated in conditions such as Alzheimer's disease, Parkinson's disease, and depression.

How does the Human BDKRB1-tGFP Stable Cell Line-CHO contribute to our understanding of BDNFR's role in synaptic plasticity?

A: The ability to visualize BDNFR activation in real-time using the GFP tag allows researchers to study the dynamics of receptor activity during synaptic plasticity events. This can help elucidate the precise timing and spatial distribution of BDNFR activation, which is crucial for understanding its role in learning and memory processes.

How does the Human BDKRB1-tGFP Stable Cell Line-CHO facilitate the study of BDNFR's interaction with other proteins in the cell?

A: The GFP tag on the BDNFR allows for the co-localization and interaction studies with other cellular proteins. Researchers can use this cell line to investigate the formation of protein complexes involving BDNFR, which can provide insights into the regulation of BDNFR signaling and its role in cellular processes.

What are the limitations of the Human BDKRB1-tGFP Stable Cell Line-CHO in mimicking the complexity of the human brain?

A: While the cell line provides a simplified model for studying BDNFR, it lacks the complexity of the human brain, including the presence of multiple cell types, the intricate neural network, and the influence of the blood-brain barrier. Therefore, findings from this cell line must be interpreted with caution and validated in more complex models or in vivo.

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