Human BDKRB1 Stable Cell Line-HEK293

Name: Human BDKRB1 Stable Cell Line-HEK293
SKU: CSC-RG0002
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0002

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-RG0002
Background	BDKRB1 gene encodes a protein that is one of two Bradykinin receptors. The bradykinin receptor family is a group of G-protein coupled receptors whose principal ligand is the protein bradykinin. This gene encodes a receptor for bradykinin B1. Bradykinin, a 9 aa peptide, is generated in pathophysiologic conditions such as inflammation, trauma, burns, shock, and allergy. The protein encoded by this gene is one of these receptors and is synthesized de novo following tissue injury. Receptor binding leads to an increase in the cytosolic calcium ion concentration, ultimately resulting in chronic and acute inflammatory responses.
Target Gene	BDKRB1
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuBDKRB1
Alias	BDKRB1, B1BKR, B1R, BKB1R, BKR1, BRADYB1
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Homo sapiens bradykinin receptor B1 DNA.
Gene Symbol	BDKRB1
Synonyms	B1R; BKR1; B1BKR; BKB1R; BRADYB1; BDKRB1
mRNA Refseq	BC034705

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What is the significance of the Human BDKRB1 Stable Cell Line-HEK293 in the study of opioid receptor function?

A: The Human BDKRB1 Stable Cell Line-HEK293 is crucial for studying the function of the mu-opioid receptor (MOR), encoded by the BDKRB1 gene. This cell line allows researchers to investigate the molecular mechanisms of opioid action, including ligand binding, signal transduction, and receptor desensitization. It provides a controlled environment for examining the effects of opioids and their analogs on receptor activity, which is essential for understanding addiction, pain management, and the development of new analgesic drugs.

How does the Human BDKRB1 Stable Cell Line-HEK293 contribute to the development of opioid addiction treatments?

A: The Human BDKRB1 Stable Cell Line-HEK293 enables researchers to study the effects of opioids on the MOR in a controlled setting. By observing how opioids interact with the receptor and cause changes in cellular signaling, scientists can identify potential targets for developing medications that mitigate the addictive properties of opioids without reducing their pain-relieving effects. This could lead to safer and more effective treatments for opioid addiction and pain management.

What are the challenges in maintaining the stability of the Human BDKRB1 Stable Cell Line-HEK293 over extended periods?

A: The Human BDKRB1 Stable Cell Line-HEK293, like any cell line, may face challenges in maintaining its genetic and phenotypic stability over time. Factors such as genetic drift, selective pressures, and culture conditions can lead to changes in the expression of the BDKRB1 gene and other cellular characteristics. Regular monitoring and quality control measures, such as DNA sequencing and functional assays, are necessary to ensure the reliability of the cell line for research purposes.

How can the Human BDKRB1 Stable Cell Line-HEK293 be used to study the pharmacokinetics of opioid compounds?

A: The Human BDKRB1 Stable Cell Line-HEK293 can be utilized in pharmacokinetic studies to evaluate the absorption, distribution, metabolism, and excretion (ADME) properties of opioid compounds. By measuring the binding affinity, efficacy, and potency of these compounds at the MOR, researchers can predict their pharmacokinetic profiles and potential therapeutic applications, which is vital for the development of new opioid drugs.

How does the Human BDKRB1 Stable Cell Line-HEK293 support the investigation of opioid receptor polymorphisms?

A: The Human BDKRB1 Stable Cell Line-HEK293 can be engineered to express different BDKRB1 polymorphisms, allowing researchers to study the functional consequences of these genetic variations on the MOR. This can provide insights into how different genetic backgrounds influence the response to opioids and may contribute to individual differences in opioid sensitivity, addiction risk, and therapeutic outcomes.

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