Psp GBD (exo-) DNA polymerase

EMQZ1411

Psp GBD (exo-) DNA Polymerase, also called Deep Vent (exo-) DNA Polymerase, has been genetically engineered to eliminate the 3´→ 5´ proofreading exonuclease activity. The half-life of Psp GBD (exo-) DNA Polymerase is 23 hours at 95°C and 8 hours at 100°C. Both Psp GBD (exo-) DNA Polymerase and Tli (exo-) DNA Polymerase are suitable for primer extensions and high temperature (72°C) DNA sequencing.

• PCR• Primer extension

2,000 U/ml

100 ul Reaction System containing: 1×Psp GBD (exo-) DNA Polymerase Buffer [20 mM Tris-HCl (pH 8.8 at 25°C),10 mM (NH4)2SO4,10 mM KCl,2 mM MgSO4,0.1% Triton X-100 (V/V)]; MgSO4 (add or not); DNA template; primers; dNTPs; 2-4 U polymerase.

100 U; 500 U

Purified from a recombinant E. coli strain that carraries Psp GBD DNA polymerase gene (D141A / E143A).

Store at -20°C

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.