Psp GBD (exo-) DNA polymerase

Name: Psp GBD (exo-) DNA polymerase
SKU: EMQZ1411
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMQZ1411

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Product Information

Cat. No.	EMQZ1411
Description	Psp GBD (exo-) DNA Polymerase, also called Deep Vent (exo-) DNA Polymerase, has been genetically engineered to eliminate the 3´→ 5´ proofreading exonuclease activity. The half-life of Psp GBD (exo-) DNA Polymerase is 23 hours at 95°C and 8 hours at 100°C. Both Psp GBD (exo-) DNA Polymerase and Tli (exo-) DNA Polymerase are suitable for primer extensions and high temperature (72°C) DNA sequencing.
Source	Purified from a recombinant E. coli strain that carraries Psp GBD DNA polymerase gene (D141A / E143A).
Concentration	2,000 U/ml
Applications	• PCR • Primer extension
Size	100 U; 500 U
Unit Definition	One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
Reaction Conditions	100 ul Reaction System containing: 1×Psp GBD (exo-) DNA Polymerase Buffer [20 mM Tris-HCl (pH 8.8 at 25°C),10 mM (NH4)2SO4,10 mM KCl,2 mM MgSO4,0.1% Triton X-100 (V/V)]; MgSO4 (add or not); DNA template; primers; dNTPs; 2-4 U polymerase.
Storage	Store at -20°C

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Customer Reviews

Customer Q&As

Does CreEasy Taq DNA Polymerase have proofreading activity?

A: The fidelity of Psp GBD DNA Polymerase partially originates from its complete 3'→5' proofreading exonuclease activity.

What is the difference between CreEasy Taq DNA Polymerase and Tli DNA Polymerase?

A: The source organism for Linkgen Psp GBD DNA Polymerase can survive at high temperatures, up to 104°C, making it more heat-stable than Tli DNA Polymerase.

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