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Goldstar Taq DNA Polymerase (2.5 U/uL,Taq Buffer, Mg2+)

For research use only. Not intended for any clinical use.
Cat.No.
EMDTS0017
Description
Goldstar Taq DNA Polymerase is provided in an inactive state with no polymerase activity at ambient temperatures. This prevents the formation of misprimed products and primer dimers at low temperatures. Goldstar Taq Polymerase is activated by a 10 minutes, 95°C incubation step, which can easily be incorporated into existing thermal cycling programs. The buffer improves specificity of a reaction, especially on GC-rich template, low copy template or template of complicated secondary structure. Goldstar Taq Polymerase catalyzes the non-template directed addition of an adenine residue to the 3′-end of both strands of DNA molecules to make it suitable for TA cloning. It has a high specificity and the PCR amplification product can be directly applied to downstream applications or chip hybridization experiment etc without the need of removing the hybrid stripes by gel recycling approach.
Applications
PCR;RT-PCR;Real-time PCR;Multiplex PCR;Microarray analysis;SNP detection;Ideal for high specificity PCR amplification
Features
`High specificity`Quick hot start: minimize the non-specific amplification products and shorten the reaction time
Note
For Research Use Only. Not for use in diagnostic procedures.
Size/Form
250U
Storage
Store at -20℃ for up to 1 year and avoid freeze thawing.

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Customer Reviews

Customer Q&As
What types of reactions can this enzyme be used for?

A: PCR;RT-PCR;Real-time PCR;Multiplex PCR;Microarray analysis;SNP detection;Ideal for high specificity PCR amplification

Can the reaction continue Can the reaction continue to be amplified after being placed at room temperature for a day after the reaction system is added?

A: Yes, Goldstar Taq DNA polymerase is provided in an inactive state and has no polymerase activity at room temperature. This prevents the formation of mis-products and primer dimers at lower temperatures.

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Customer Reviews
High specificity

Highly specific fast hot start, minimizes non-specific amplification products, and shortens reaction times, especially on GC-rich templates, low-copy templates, or templates with complex secondary structures.

United States

12/17/2022

Easy to use after amplification

PCR amplification products can be used directly in downstream applications or microarray hybridization experiments, etc., without removing hybridization streaks by gel recovery methods.

United States

01/19/2023

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