GC-Rich DNA Polymerase

Name: GC-Rich DNA Polymerase
SKU: EMDTS0020
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMDTS0020

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Product Information

Cat. No.	EMDTS0020
Description	GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets or non-GC-rich cDNA, plasmid, and lambda-based targets.
Features	Yield - High yields for targets up to 5 kb in length Specificity - AccuPrime accessory proteins for improved PCR specificity Sensitivity - Sensitivity down to 5 ng of template DNA
Applications	The buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.
Size	200 reactions, 1000 reactions
Unit Definition	One unit of GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min. at 74°C.
Note	For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Storage	Store at -20℃
Shipping	Approved for shipment on Wet or Dry Ice

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Customer Reviews

Customer Q&As

Is the enzyme highly specific?

A: The buffer solution in this product contains a thermostable protein that enhances the hybridization between the primer and template during PCR, leading to high specificity.

Can templates with GC content greater than 65% be amplified?

A: Yes, this product can provide high yield and high specificity amplification for templates with GC content >65%.

Can the kit be used to amplify non-GC-rich cDNA?

A: Sure, this product provides multiple buffer options, which can be selected based on the specific needs.

Does it work well for amplification of long fragment templates?

A: The result is very good, and it has high yield even for templates with a length of up to 5kb.

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Customer Reviews

Fast synthesis speed

The synthetic ability of the product is five times higher than that of Taq DNA polymerase.

United States

2022-03-25

Strong thermal stability

The enzyme still maintains good activity after 4 hours at 95°C.

United States

2022-06-16

High sensitivity

When amplifying cDNA, the enzyme has a sensitivity as low as 5ng of template DNA.

United States

2023-01-12

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GC-Rich DNA Polymerase

Product Information

Q & A

Customer Reviews

Is the enzyme highly specific?

Can templates with GC content greater than 65% be amplified?

Can the kit be used to amplify non-GC-rich cDNA?

Does it work well for amplification of long fragment templates?

Fast synthesis speed

Strong thermal stability

High sensitivity

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