GC-Rich DNA Polymerase

EMDTS0020

GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets or non-GC-rich cDNA, plasmid, and lambda-based targets.

The buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.

Yield - High yields for targets up to 5 kb in length Specificity - AccuPrime accessory proteins for improved PCR specificity Sensitivity - Sensitivity down to 5 ng of template DNA

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Approved for shipment on Wet or Dry Ice

200 reactions, 1000 reactions

Store at -20℃

One unit of GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min. at 74°C.