Transfected Stable Cell Lines
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Cat. No. : EDK-01
| Cat. No. | EDK-01 |
| Description |
Chromogenic LAL Endotoxin Assay Kit is used to in vitro end-point endotoxin quantitative test of human and animal parenteral drugs, biological products, and medical devices. The kit utilizes a modified Limulus Amebocyte Lysate and a synthetic color producing substrate to detect endotoxin chromogenically, detecting concentrations ranging from 0.01 - 1 EU/ml. Note: any sample with color (e.g. bacterial /cell culture medium, serum or blood etc.) cannot be assayed using this kit. |
| Features |
· Highly sensitive · Ready-to-use · Fast and reliable · Wide range of applications |
| Applications | In vitro end-point endotoxin test |
| Storage |
The kit can be stored dry at room temperature for up to 1 month. For long-term use, the kit can be kept at 2-8°C for up to one year. Note: do not freeze the kit or any of its components. |
A: The LAL (limulus amebocyte lysate) testing, also known as bacterial endotoxin testing, is an in vitro assay used to detect the presence and concentration of bacterial endotoxins in drugs and biological products, and is an important part of pharmaceutical microbiology.
A: The chromogenic LAL assay is a quantitative test that produces a yellow colour (chromogenic) as the result of an enzymatic reaction triggered by the activation of limulus clotting factor C by the presence of bacterial endotoxin in a sample. Within a kit specific range, the colour intensity is directly proportional to the quantity of contaminants in the sample: the more endotoxin, the stronger yellow the solution will become. The chromogenic LAL reaction can be quantified by absorbance detection using a spectrophotometer or an absorbance microplate reader.
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The incubation period can be shortened to 14 minutes and color-stabilizer ensures accurate results.
Detect endotoxin concentration in the range of 0.01 - 1 EU/ml. And assay requires only 10μL of a protein sample.
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