Cat.No. : VNV-036
Storage: -80°C Shipping: Dry ice
| Cat. No. | VNV-036 |
| Description | Wild type zika virus (strain Uganda MP1751) which are inactivated by heat treatment. This product is intended for research use only. |
| Shipping | Dry ice |
| Storage | -80°C |
| Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. | |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
Zika virus (ZIKV) causes congenital Zika syndrome, including various alterations in central nervous system development. Although infection of cells of the nervous system has been well documented, little is known about the ability of Zika virus to infect immune cells. Here, researchers investigated whether freshly isolated peripheral blood mononuclear cells (PBMCs) from healthy donors could be infected by Zika virus. They detected Zika virus RNA in PBMCs from all donors by RT-qPCR analysis. Detection of viral antigens by flow cytometry revealed that more than 50% of the donors' PBMCs were infected with Zika virus, with CD3+CD4+ T cells, CD3−CD19+ B cells, and CD3+CD8+ T cells being the most frequently infected subsets, respectively, followed by CD14+ monocytes. Furthermore, there was a wide variation in the infection rate of PBMCs between different donors, both in terms of the number and type of infected cells. These findings raise the hypothesis that PBMCs could act as a reservoir for the virus, which could facilitate viral dissemination to different organs, including immune-privileged sites.
To verify whether the detected viral loads were due to active PBMC infection rather than passive uptake of viral particles, the researchers performed viral titrations in culture supernatants. At 24, 48, and 72 hours post-infection, supernatants from MOCK, inactivated ZIKV, or ZIKV-infected PBMCs were harvested and used to infect Vero cell monolayers. The results showed that viral titrations increased over time, indicating active PBMC infection. The researchers were unable to detect viral particles in the supernatants of MOCK or inactivated ZIKV-infected samples (Figure 1A). This result also suggests that viral particles produced by PBMCs can infect other cells, as the titrations were performed in Vero cells (Figure 1B). Overall, these data clearly indicate that human PBMCs are a target of ZIKV, at least under the experimental conditions applied in this study.
Figure 1. ZIKV viral multiplication in PBMCs. (Messias C V, et al., 2019)
A: Zika virus is an emerging disease that is spread by Aedes mosquitoes. The virus was first isolated in Central Africa, and has since been spread to South Asia and recently to South America. Zika virus can cause mild fever, rash, myalgia, arthralgia and headaches, with one in four infected individuals being asymptomatic.
A: Yes.Store at -80°C is recommended. Avoid multiple freeze/thaw cycles.
A: Zika virus (ZIKV) is a member of the Flaviviridae family of viruses. It is transmitted by Aedes mosquitoes that are active during the day, such as A. aegypti and A. albopictus.
A: Symptoms of Zika virus infection are usually mild and include fever, rash, joint pain, and conjunctivitis.
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The product has tested and quantified infectivity and is a good laboratory material for infectious disease research in our laboratory.
Wild-Type Zika Virus (strain Uganda MP1751) provided by Creative Biogene has been whole genome sequenced for reliable quality assurance. I recommend purchasing it.
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