Cat.No. : VNV-033
Storage: -80°C Shipping: Dry ice
| Cat. No. | VNV-033 |
| Description | Wild-type varicella zoster viruses which are prepared from cell cultures and are inactivated by β-Propiolactone treatment. This product is intended for research use only. |
| Shipping | Dry ice |
| Storage | -80°C |
| Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. | |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
CD4+ T cells are an essential component of an effective immune response against varicella-zoster virus (VZV), but their functional properties during both the acute and latent phases of infection reactivation remain poorly understood. Here, researchers found significant differences between the polyfunctionality of VZV-specific total memory, effector memory, and central memory CD4+ T cells in acute and prior acute herpes zoster (HZ). The frequencies of IFN-γ and IL-2-producing cells were higher in VZV-specific CD4+ memory T cell responses in acute HZ reactivation compared with prior HZ. Furthermore, cytotoxic markers were elevated in VZV-specific CD4+ T cells compared with non-VZV-specific cells. Transcriptome analysis of ex vivo total memory CD4+ T cells from these individuals revealed differential regulation of T cell survival and differentiation pathways, including the TCR, cytotoxic T lymphocyte (CTL), T helper, inflammatory, and MTOR signaling pathways. These gene signatures correlated with the frequencies of IFN-γ and IL-2-producing cells in response to VZV.
To initially characterize the nature of VZV-specific CD4+ T cell responses during prior and acute reactivation HZ, blood samples were collected from individuals with acute HZ due to VZV reactivation (n=29) (acute HZ group) and individuals with a history of HZ (n=16) (prior HZ group). After PBMCs were stimulated overnight with whole inactivated VZV or mitogens, differences in T cell memory distribution and VZV-specific CD4+ and CD8+ T cell responses were assessed. The study showed that VZV-induced cytokine-producing CD4+ T cells contained more than twice the number of EM cells, while the frequency of cytokine-producing CM cells was significantly lower (Figure 1). In the EM population, the frequencies of TNF-α+, IL-2+, and IFN-γ+ cells were significantly higher in patients with acute herpes zoster than in patients with previous herpes zoster (P<0.05, P<0.05, and P<0.01, respectively). These results suggest that during the acute phase of herpes zoster, viral control is primarily performed by EM T cells.
Figure 1. Violin plots for cytokine positive T-cell percentages under VZV stimulation in total memory CD4+ T cells. (Jin W, et al., 2022)
A: Passage the virus by co-infection of fresh cells. Thaw ampoule in 37°C water bath. Use virus to co-infect cells in a T25 flask for first passage. Multiple passages may enhance the titer. Preserve as you would live cells.
A: 4-7 days.
A: The wild type varicella zoster virus is a type of herpesvirus that causes chickenpox (varicella) and can later reactivate to cause shingles (herpes zoster) in some individuals.
A: Yes, the wild type varicella zoster virus we provide are safe for use in research, as they are produced under stringent quality control measures and comply with applicable regulations. Researchers should follow appropriate biosafety protocols and handle these products in certified laboratories.
A: Yes, our Company often offers customization options for the wild type varicella zoster virus products, allowing researchers to tailor the products based on their specific research requirements. It is recommended to contact the Company's customer support or sales team for further information regarding customization options.
A: Varicella zoster virus (VZV), also known as human herpesvirus 3 (HHV-3, HHV3) or Human alphaherpesvirus 3 (taxonomically), is one of nine known herpes viruses that can infect humans.
A: The genome of Varicella Zoster Virus was first sequenced in 1986. It is a linear double-stranded DNA molecule, and the laboratory strain has 124,884 base pairs.
A: Varicella zoster virus (VZV) virions are spherical in shape, with a diameter of 180-200 nm. Their lipid envelope surrounds a 100 nm nucleocapsid of 162 hexameric and pentameric capsomers arranged in an icosahedral pattern.
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This product is an important tool for disease research, and we have successfully used it to study Varicella Zoster virus infections and diseases.
The ease of use and clear instructions provided with the products have made our experiments run smoothly. I highly recommend these products for anyone conducting research on the Varicella Zoster Virus.
This product has greatly contributed to advancing our understanding of Varicella Zoster Virus interactions with host immune cells. I commend your company for providing such reliable and valuable research tools.
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