Cat.No. : VNV-004
Storage: -80°C Shipping: Dry ice
| Cat. No. | VNV-004 |
| Description | These viruses are wild type human adenovirus serotype 40 particles which are replication-competent. This product is intended for research use only. |
| Shipping | Dry ice |
| Storage | -80°C |
| Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. | |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
A: To avoid contamination during the activation of Wild-Type Human Adenovirus (Serotype 40), it is crucial to follow strict aseptic techniques and precautions. Firstly, the preparatory work area should be thoroughly cleaned and disinfected before starting any procedures. All equipment, including pipettes, tubes, and bottles, should be sterile and handled with sterile gloves. It is essential to wear appropriate personal protective equipment such as lab coats, goggles, and masks to prevent any potential aerosol contamination. Additionally, working in a biosafety cabinet with appropriate containment levels is recommended to minimize the risk of any accidental release of the virus. All procedures should be carried out carefully and slowly to minimize the generation of any splashes or aerosols. Proper waste management and disposal should be practiced, using autoclaving or other appropriate methods for the safe inactivation of any virus-containing waste. Regular monitoring and testing of laboratory surfaces, equipment, and personnel can also be implemented to ensure the absence of any contamination. Vigilance, adherence to protocols, and ongoing training are essential to avoid contamination during the activation of Wild-Type Human Adenovirus (Serotype 40).
A: For the safety of the Wild-Type Human Adenovirus (Serotype 40) study, several recommendations should be followed. First, proper containment measures should be implemented to prevent accidental release or exposure of the virus to the environment or individuals. This includes adhering to strict laboratory protocols, using appropriate personal protective equipment, and conducting the study in a Biosafety Level 2 or higher containment facility. Additionally, thorough risk assessments should be performed to assess the potential hazards associated with the study and necessary mitigations should be implemented accordingly. Regular monitoring of the study site and participants should be employed to ensure early detection of any adverse events or infections, followed by immediate implementation of appropriate quarantine measures if required. Staff involved in the study should receive comprehensive training on handling the virus, understanding its potential risks, and following all safety protocols. Overall, by strictly adhering to these safety measures, the risk associated with the Wild-Type Human Adenovirus study can be minimized, ensuring the well-being of both researchers and participants.
A: Adenovirus refers to a group of viruses that can cause a range of illnesses, including the common cold, respiratory infections, conjunctivitis (pink eye) and gastrointestinal infections. There are more than 50 different types of adenovirus, divided into seven types (A to G).
A: Enteric adenovirus 40 has been recognized as the second most important causative agent of viral gastroenteritis in children. In contrast to other adenoviruses, these viruses are not shed in respiratory secretions; therefore, their transmission is limited to the oral-fecal route.
A: Symptoms may include fever, diarrhea, vomiting and abdominal pain lasting about 10 days. Some people may experience respiratory symptoms.
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Our research team has been relying on Wild-Type Human Adenovirus (Serotype 40) from Creative Biogene for quite some time, and we are extremely satisfied with their offerings.
The prompt delivery and responsive customer service have made our research progress smooth and efficient. We highly recommend these products to any researcher aiming to deepen their understanding of Adenovirus (Serotype 40).
We were particularly impressed by the high viral titer and purity of the Wild-Type Human Adenovirus (Serotype 40). This level of quality ensured that our infection assays were both efficient and effective, saving us valuable time and resources.
The Wild-Type Human Adenovirus (Serotype 40) product exceeded our lab's expectations with its remarkable consistency and reliability. Each batch delivered reproducible results, making our virology experiments much more streamlined and dependable.
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