Cat.No. : VNV-012
Storage: -80°C Shipping: Dry ice
| Cat. No. | VNV-012 |
| Description | Wild-type herpes simplex virus 1 (HSV-1, strain: 17) which are prepared from host cells and are inactivated using Triton X-100. This product is intended for research use only. |
| Shipping | Dry ice |
| Storage | -80°C |
| Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. | |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
Herpes labialis are a common disease characterized by blisters or erosions on the lips and skin around the mouth and nose. Most cases are caused by herpes simplex virus type 1 (HSV-1), but 10-15% are caused by HSV-2. Studies have shown that the frequency and severity of HSV-1 outbreaks are thought to depend on the effectiveness of immune control of the virus. McKenna showed that people with frequent outbreaks had lower lymphocyte proliferation in vitro in the presence of inactivated HSV-1 and lower levels of interferon-gamma protein in vitro in the presence of inactivated HSV-1 compared with people with infrequent outbreaks. Here, the researchers sought to determine differences in overall immune function, and specifically immune responses to HSV-1, between people infected with HSV-1 and who frequently develop herpes labialis, and people infected with HSV-1 and who do not frequently develop herpes labialis, or people infected with HSV-1 and who have never developed herpes labialis.
The study showed that those with good immune control of HSV-1 infection (fewer outbreaks) differed from those with poor immune control in the following ways: (1) PBMCs had greater proliferation in vitro to HSV-1, HSV-1 infected cell extracts, and Candida species (P < 0.01). (2) PBMCs stimulated in vitro with HSV-1 virus had higher expression of IFNG and five other immune-related genes (each P < 0.05) and lower expression of IL5 and two other immune-related genes (each P < 0.05). Overall, higher expression levels of IFNG and lower expression levels of IL5 in PBMCs were associated with fewer herpes labialis episodes in the presence of HSV-1, and a single topical application of SADBE to the arm of subjects with frequent herpes labialis episodes improved the immune response to HSV-1.
Figure 1. PBMC normalized proliferation in the three test stimuli taken together (HSV-1-infected cell extracts, inactivated HSV-1, and Candida extract). (McTavish H, et al., 2019)
A: Wild-Type HSV-1 refers to the original, unmodified form of the herpes simplex virus type 1. It is used for research to understand the virus's biology, pathogenesis, replication, and the development of therapeutics and vaccines against HSV-1.
A: Wild-Type HSV-1 products are often used to infect cell cultures for investigating viral replication, gene expression, and the cellular response to infection. They can also be used to study viral entry mechanisms, the establishment of latency, and the development of antiviral drugs.
A: Herpes simplex is a virus that causes skin infections. This infection can last a lifetime and cause painful or itchy sores and blisters. Herpes simplex virus usually does not cause serious problems. However, it can be dangerous for babies and people with weakened immune systems.
A: HSV-1 mostly spreads by oral contact and causes infections in or around the mouth (oral herpes or cold sores). HSV-2 spreads by sexual contact and causes genital herpes.
A: The HSV genome spans approximately 150,000 bp and consists of two unique segments, termed unique long (UL) and unique short (US), and terminal inverted repeats found at either end of them, termed repeat long (RL) and repeat short (RS).
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The reliability and consistency of the virus strain have been exceptional, allowing me to conduct my experiments with confidence.
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