Cat.No. : VNV-011
Storage: -80°C Shipping: Dry ice
| Cat. No. | VNV-011 |
| Description | Wild type dengue viruses (Type 2) which are inactivated by heat treatment. This product is intended for research use only. |
| Shipping | Dry ice |
| Storage | -80°C |
| Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. | |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
Infection with dengue virus (DENV) results in increased proinflammatory responses, such as nitric oxide (NO) production and TNF-α expression. The studies here show that in addition to TNF-α production in DENV-infected mouse macrophage RAW264.7 cells, inducible NO synthase is also elevated transcriptionally and translationally, accompanied by NO production. NF-κB is known to be activated by DENV infection. Pharmacological inhibition of NF-κB activation abolished iNOS/NO biosynthesis and TNF-α production. By inhibition, the potential role of NF-κB in the regulation of oxidative signaling during DENV infection was prevented. Heat-inactivated DENV (Inactivated Wild-Type Dengue Virus) did not induce the identified inflammatory response. Pharmacological inhibition of TLR3 partially reduced NF-κB activation. However, it effectively abolished inducible iNOS/NO biosynthesis but did not inhibit TNF-α production. In contrast to TLR3, the viral protein NS2B3 also independently promotes NF-κB activation to regulate TNF-α production. These results show distinct pathways of NF-κB activation induced by DENV infection, regulating the expression of iNOS/NO and TNF-α, respectively.
To investigate whether DENV-induced NF-κB activation, followed by TNF-α and iNOS/NO biosynthesis, occurs in a protein-mediated manner, RAW264.7 cells were inoculated with heat-inactivated DENV (HI-DENV) at an MOI of 50. The results showed that HI-DENV induced NF-κB activity less efficiently than live DENV (Figure 1). Similarly, the expression levels of TNF-α, NO, and iNOS were lower in the HI-DENV group compared with the live DENV group. These experiments suggest that DENV activates NF-κB through viral proteins, followed by TNF-α and iNOS/NO biosynthesis.
Figure 1. Heat-inactivated DENV does not efficiently cause NF-κB activation. (Cheng Y L, et al., 2015)
A: Wild-type Dengue Virus are strains of the virus that closely resemble the naturally occurring strains found in the population. By using these products, researchers can better understand the behavior, replication, and immune response of the virus in real-world scenarios.
A: Yes, wild-type Dengue Virus are instrumental in studying the immune response to the virus. Researchers can use these products to investigate the production of antibodies, cytokines, and other immune markers in infected individuals or experimental models, thus providing valuable insights into the host immune response.
A: Dengue virus (DENV) is the cause of dengue fever. It is a mosquito-borne single positive-strand RNA virus of the family Flaviviridae; genus Flavivirus.
A: The Dengue Virus genome consists of approximately 11,000 bases of positive-sense single-stranded RNA (ssRNA) encoding three structural proteins (capsid protein C, membrane protein M, and envelope protein E) and seven non-structural proteins (NS1, NS2a, NS2b , NS3, NS4a, NS4b, NS5). It also contains short non-coding regions at both the 5' and 3' ends.
A: Symptoms of dengue virus infection include high fever, severe headache, joint and muscle pain, rash, nausea and vomiting. In severe cases, it can lead to dengue hemorrhagic fever or dengue shock syndrome, which can be life-threatening.
A: There are four distinct serotypes of the Dengue Virus, named DENV-1, DENV-2, DENV-3, and DENV-4.
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I have been using the Wild-Type Dengue Virus products for my research on antiviral drug development, and I am extremely satisfied with the quality of these products.
The virus provided by Creative Biogene are highly accurate representations of the Wild-Type Dengue Virus, enabling us to obtain reliable and reproducible results.
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