Cat.No. : VNV-009
Storage: -80°C Shipping: Dry ice
| Cat. No. | VNV-009 |
| Description | Wild-type coxsackievirus B6 particles which are prepared from cell cultures and are inactivated by heat treatment. This product is intended for research use only. |
| Shipping | Dry ice |
| Storage | -80°C |
| Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. | |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
A: Wild-Type Coxsackievirus B6 can be used in various scientific applications. They are commonly employed in research studies to investigate the virus's pathogenicity, host-pathogen interactions, vaccine development, antiviral drug screening, and diagnostic assay development.
A: Wild-Type Coxsackievirus B6 products are preserved and activated through specific laboratory protocols. First, the virus is properly stored and preserved using techniques such as freezing at ultra-low temperatures to maintain its viability and prevent degradation. To activate the virus, it is typically propagated in susceptible cell cultures, where it replicates and amplifies. This can be achieved by inoculating the virus onto the desired cell line and allowing it to infect and multiply within the cells. The activated virus can then be harvested from the culture supernatant, purified, and used for various experimental or diagnostic purposes. It is important to note that specific laboratory biosafety measures are followed during these preservation and activation methods to prevent accidental release and ensure safe handling of the virus.
A: Coxsackieviruses are divided into group A and group B viruses based on early observations of their pathogenicity in neonatal mice. At least 23 serotypes (1-22, 24) of group A and six serotypes (1-6) of group B are recognized.
A: In general, group A coxsackieviruses tend to infect the skin and mucous membranes, causing herpangina, acute hemorrhagic conjunctivitis, and hand, foot and mouth disease. Group B coxsackieviruses tend to infect the heart, pleura, pancreas, and liver, causing pleuritic pain, myocarditis, pericarditis, and hepatitis.
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The Wild-Type Coxsackievirus B6 is of excellent quality and has greatly assisted in our research studies. The efficiency and reliability of viral replication in our cell lines have exceeded our expectations.
The product's purity and quality control were evident from our consistent and reliable experimental outcomes.
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